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dc.creatorJanković, Goran
dc.creatorMarinko, Marija
dc.creatorMilojević, Predrag
dc.creatorStojanović, Ivan
dc.creatorNenezić, Dragoslav
dc.creatorKanjuh, Vladimir
dc.creatorYang, Qin
dc.creatorHe, Guo-Wei
dc.creatorNovaković, Aleksandra
dc.date.accessioned2020-02-18T10:15:39Z
dc.date.available2020-02-18T10:15:39Z
dc.date.issued2019
dc.identifier.issn1347-8613
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3515
dc.description.abstractCardioprotective abilities of procyanidins, might, at least in part, attribute to their vasodilator properties. The present study was undertaken to assess the vasorelaxant effect of procyanidin B2 on isolated human saphenous vein (HSV) and its underlying mechanisms. Procyanidin B2 relaxed phenylephrine-induced contraction of HSV rings in concentration-dependent manner. The relaxation was dependent on the presence of endothelium and was strongly affected by l-NAME, hydroxocobalamin or ODQ, the inhibitors of NO/cGMP pathway. Indomethacin significantly affected only the relaxation produced by the highest concentrations of procyanidin B2. Apamin and TRAM-34 combination, in the presence of l-NAME and indomethacin, did not additionally decreased procyanidin B2-induced relaxation. In the presence of K+ channel blockers, relaxation induced by procyanidin B2 was partially attenuated by 4-aminopyridine, significantly inhibited by glibenclamide and almost abolished by iberiotoxin. Procyanidin B2 also relaxed the contractions induced by phenylephrine or caffeine in Ca2+-free solution. Finally, nifedipine slightly, while thapsigargin strongly antagonized HSV relaxation. Our results indicate that procyanidin B2 induces endothelium-dependent relaxation of HSV, which results primarily from stimulation of NO production, as well K+ channels opening, especially BKCa, and partially KATP and KV. Regulation of the intracellular Ca2+ release and inhibition of Ca2+ influx probably contr
dc.publisherElsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175088/RS//
dc.rightsopenAccess
dc.sourceJournal of Pharmacological Sciences
dc.subjectCa2+ channels
dc.subjectEndothelium
dc.subjectHuman saphenous vein
dc.subjectK+ channels
dc.subjectProcyanidin B2
dc.subjectVasorelaxation
dc.titleMechanisms of endothelium-dependent vasorelaxation induced by procyanidin B2 in venous bypass graft
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЈанковић, Горан; Милојевић, Предраг; Стојановић, Иван; Ненезић, Драгослав; Новаковић, Aлександра; Маринко, Марија; Кањух, Владимир; Yанг, Qин; Хе, Гуо-Wеи;
dc.citation.volume142
dc.citation.issue3
dc.citation.spage101
dc.citation.epage108
dc.citation.rankM22
dc.identifier.wos000513775600003
dc.identifier.doi10.1016/j.jphs.2019.11.006
dc.identifier.scopus2-s2.0-85076843938
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/7519/Mechanisms_of_endothelium_pub_2019.pdf
dc.type.versionpublishedVersion


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