Increased plasma phosphatidylcholine/lysophosphatidylcholine ratios in patients with Parkinson's disease
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AuthorsMiletić Vukajlović, Jadranka
Ilić, Tihomir V.
Article (Published version)
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Rationale: Changes in lipid composition might be associated with the onset and progression of various neurodegenerative diseases. Herein, we investigated the changes in the plasma phosphatidylcholine (PC)/lysophosphatidylcholine (LPC) ratios in patients with Parkinson's disease (PD) in comparison with healthy subjects and their correlation with clinico-pathological features. Methods: The study included 10 controls and 25 patients with PD. All patients were assigned to groups based on clinico-pathological characteristics (gender, age at examination, duration of disease and Hoehn and Yahr (H&Y) stage). The analysis of the PC/LPC intensity ratios in plasma lipid extracts was performed using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Results: PD patients exhibited an increased PC/LPC intensity ratio in comparison with the control group of healthy subjects. Furthermore, the investigated ratio was shown to be correlated with clinico-pathological parameters,... in particular with H&Y stage and disease duration. The PC/LPC intensity ratio in plasma samples of PD patients was found to be elevated in all examined H&Y stages and throughout the disease duration. Conclusions: To our knowledge, this is the first study examining the PC/LPC ratios in plasma of patients with PD and illustrating their correlation with clinico-pathological features. Although the presented results may be considered as preliminary due to the limited number of participants, the observed alterations of PC/LPC ratios in plasma might be a first step in the characterization of plasma lipid changes in PD patients and an indicator of lipid reconfiguration.
Keywords:MALDI-TOF MS / PHOSPHOCHOLINE CYTIDYLYLTRANSFERASE ACTIVITY / PHOSPHOLIPASE A(2) ACTIVITY / LIPID-PEROXIDATION / MASS-SPECTROMETRY / O-ACYLTRANSFERASE / SUBSTANTIA-NIGRA / OXIDATIVE STRESS / FATTY-ACID / LYSOPHOSPHATIDYLCHOLINE
Source:Rapid Communications in Mass Spectrometry, 2020, 34, 4
- John Wiley and Sons Ltd