The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E.
Fröhner), A. fissa Günther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and
quercetin-3-O-β-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts
were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 μg/mL for methanol extracts of
A. viridiflora, A. glabra and A. monticola, and 256 μg/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was
obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species
(0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4 -32 μg.../mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5
μg/mL), while the second identified compound, quercetin-3-O-β-D-glucoside, was also very active in concentration of 2-16 μg/mL.
This is peer-reviedversion ofg the folloving article: (1) Krivokuća, M.; Niketić, M.; Milenković, M.; Golić, N.; Masia, C.; Scaltrito, M. M.; Sisto, F.; Kundaković, T. Anti-Helicobacter Pylori Activity of Four Alchemilla Species (Rosaceae). Nat. Pro. Comm. 2015, 10 (8), 1369–1371.
TY - JOUR
AU - Krivokuća, Marija
AU - Niketić, Marjan
AU - Milenković, Marina
AU - Golić, Nataša
AU - Masia, Carla
AU - Scaltrito, Maria Maddalena
AU - Sisto, Franceska
AU - Kundaković, Tatjana
PY - 2015
UR - [http://farfar.pharmacy.bg.ac.rs/handle/123456789/3537]
AB - The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E.
Fröhner), A. fissa Günther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and
quercetin-3-O-β-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts
were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 μg/mL for methanol extracts of
A. viridiflora, A. glabra and A. monticola, and 256 μg/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was
obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species
(0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4 -32 μg/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5
μg/mL), while the second identified compound, quercetin-3-O-β-D-glucoside, was also very active in concentration of 2-16 μg/mL.
PB - Natural Products Inc, Westerville
T2 - Natural Product Communications
T1 - Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)
VL - 10
UR - https://hdl.handle.net/21.15107/rcub_farfar_3537
ER -
@article{
author = "Krivokuća, Marija and Niketić, Marjan and Milenković, Marina and Golić, Nataša and Masia, Carla and Scaltrito, Maria Maddalena and Sisto, Franceska and Kundaković, Tatjana",
year = "2015",
abstract = "The present study evaluated the anti-Helicobacter pylori activity of Alchemilla glabra Neygenf. (A. sect. Alchemilla), A. monticola Opiz (A. sect. Plicatae S.E.
Fröhner), A. fissa Günther & Schummel (A. sect. Calycinae (Buser) Buser) and A. viridiflora Rothm. (A. sect. Calycinae), and identified ellagic acid and
quercetin-3-O-β-D-glucoside. Anti-H. pylori activity was tested against ten clinical isolates and one reference strain (ATCC 43504). The methanol extracts
were more active than the dichloromethane and cyclohexane extracts. The ranges of concentrations were between 4 μg/mL for methanol extracts of
A. viridiflora, A. glabra and A. monticola, and 256 μg/mL for cyclohexane extracts of A. viridiflora, A. glabra and A. fissa. The best overall activity was
obtained with A. monticola extracts. No significant difference was found in the ellagic acid contents of the methanol extracts of the tested Alchemilla species
(0.2-0.3 mg/mL), and anti-H. pylori activity was similar (4 -32 μg/mL). Ellagic acid exhibited strong activity at very low concentrations (0.125-0.5
μg/mL), while the second identified compound, quercetin-3-O-β-D-glucoside, was also very active in concentration of 2-16 μg/mL.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)",
volume = "10",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3537"
}
Krivokuća, M., Niketić, M., Milenković, M., Golić, N., Masia, C., Scaltrito, M. M., Sisto, F.,& Kundaković, T.. (2015). Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications
Natural Products Inc, Westerville., 10.
https://hdl.handle.net/21.15107/rcub_farfar_3537
Krivokuća M, Niketić M, Milenković M, Golić N, Masia C, Scaltrito MM, Sisto F, Kundaković T. Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae). in Natural Product Communications. 2015;10.
https://hdl.handle.net/21.15107/rcub_farfar_3537 .
Krivokuća, Marija, Niketić, Marjan, Milenković, Marina, Golić, Nataša, Masia, Carla, Scaltrito, Maria Maddalena, Sisto, Franceska, Kundaković, Tatjana, "Anti-Helicobacter pylori Activity of Four Alchemilla Species (Rosaceae)" in Natural Product Communications, 10 (2015),
https://hdl.handle.net/21.15107/rcub_farfar_3537 .
