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dc.creatorMunjas, Jelena
dc.creatorSopić, Miron
dc.creatorJoksić, Ivana
dc.creatorProsenc Zmrzljak, Ursula
dc.creatorKaradžov-Orlić, Nataša
dc.creatorKošir, Rok
dc.creatorEgić, Amira
dc.creatorMiković, Željko
dc.creatorNinić, Ana
dc.creatorSpasojević-Kalimanovska, Vesna
dc.date.accessioned2020-06-12T10:06:33Z
dc.date.available2020-06-12T10:06:33Z
dc.date.issued2020
dc.identifier.issn0009-9120
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3602
dc.description.abstractIntroduction: MicroRNAs have a significant role in the pathogenesis of preeclampsia. Circulating microRNAs could represent a potential biomarker for preeclampsia. The aim of this study was to evaluate plasma miR210-3p and miR518b in preeclampsia and healthy pregnancy for the first time by digital droplet PCR (ddPCR). Methods: Thirty-six pregnant women (seventeen healthy pregnancies, nineteen preeclampsia patients) were involved from the Clinic for Gynaecology and Obstetrics “Narodni front” in Belgrade, Serbia. Plasma miR210-3p, miR518b and cel-miR-39 as a spike-in control were measured by ddPCR. Results: MiR518b was significantly elevated in preeclampsia compared to a healthy pregnancy (P = 0.034; 0.302(0.217–0.421) vs. 0.171(0.110–0.266)). MiR210-3p showed no significant difference between the two groups (P = 0.951). The adjustment of miR518b was made for a gestational age and smoking status and the difference between the preeclampsia and healthy pregnancy group was more significant (P = 0.026; 0.300(0.216–0.419) vs. 0.172(0.121–0.245)). Plasma miR-518b was significantly higher in the group of preeclampsia patients with proteinuria above the 75th percentile for the group (P=0.033), in women who smoked (P=0.039), and was positively related to uric acid in preeclampsia (P = 0.018, r = 0.536). Plasma miR518b was able to significantly discriminate between preeclampsia and healthy pregnancy, yielding AUC of 0.712 (95%CI:0.539–0.891), P = 0.028. Conclusions: In this study plasma microRNA were measured for the first time in preeclampsia and healthy pregnancies with ddPCR. Placenta-specific miR-518b could serve as a potential biomarker for discriminating preeclampsia and healthy pregnancy, which should be confirmed on a larger study population. This study has failed to confirm the same potential for miR210-3p.
dc.publisherElsevier
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/175035/RS//
dc.relationddPCR Grant challenge initiative from Labena Ltd., Slovenia
dc.rightsrestrictedAccess
dc.sourceClinical Biochemistry
dc.subjectDigital droplet PCR
dc.subjectmiR210-3p
dc.subjectmiR518b
dc.subjectPreeclampsia
dc.titlePlacenta-specific plasma miR518b is a potential biomarker for preeclampsia
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМуњас, Јелена; Сопић, Мирон; Јоксић, Ивана; Егић, Aмира; Миковић, Жељко; Нинић, Aна; Спасојевић-Калимановска, Весна; Просенц Змрзљак, Урсула; Караджов-Орлић, Наташа; Кошир, Рок; Плацента-специфиц пласма миР518б ис а потентиал биомаркер фор преецлампсиа;
dc.citation.volume79
dc.citation.spage28
dc.citation.epage33
dc.identifier.wos000530708900005
dc.identifier.doi10.1016/j.clinbiochem.2020.02.012
dc.identifier.scopus2-s2.0-85081737467
dc.type.versionpublishedVersion


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