dc.creator | Rupar, Jelena | |
dc.creator | Aleksić, Mara | |
dc.creator | Dobričić, Vladimir | |
dc.creator | Brborić, Jasmina | |
dc.creator | Čudina, Olivera | |
dc.date.accessioned | 2020-06-17T08:17:22Z | |
dc.date.available | 2020-06-17T08:17:22Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 1567-5394 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3611 | |
dc.description.abstract | The electrochemical behavior of 9-chloroacridine (9Cl-A), a precursor molecule for synthesis of acridine derivatives with cytostatic activity, is a complex, pH-dependent, diffusion-controlled irreversible process. Oxidation of 9Cl-A initiates with the formation of a cation radical monomer, continues via the formation of a dimer subsequent oxidation to new cation radical. Reduction of 9Cl-A produces radical monomers which are stabilized by dimer formation. The investigation was performed using cyclic, differential pulse and square wave voltammetry at a glassy carbon electrode. The interaction between 9Cl-A and double-stranded DNA (dsDNA) was investigated using a multilayer dsDNA-electrochemical biosensor and 9Cl-A solutions from 1.0×10-7M (the lowest 9Cl-A concentration whose interaction with DNA was possible to detect) up to 1×10-4M. These allowed the binding constant, K=3.45×105M-1 and change in Gibbs free energy of the formed adsorbed complex to be calculated. Complex formation was a spontaneous process proceeding via 9Cl-A intercalation into dsDNA inducing structural changes. The intercalation of 9Cl-A into dsDNA was supported by molecular docking analysis. The combination of simple methodology and the use of biosensors to investigate DNA interactions is a powerful tool to offer insight into aspects of drug design during pharmaceutical development. | en |
dc.publisher | Elsevier B.V. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172033/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/172041/RS// | |
dc.rights | restrictedAccess | |
dc.source | Bioelectrochemistry | |
dc.subject | 9-Chloroacridine redox mechanism | |
dc.subject | dsDNA-electrochemical biosensor | |
dc.subject | Interaction | |
dc.subject | Molecular docking | |
dc.subject | Square wave voltammetry | |
dc.title | An electrochemical study of 9-chloroacridine redox behavior and its interaction with double-stranded DNA | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Добричић, Владимир; Aлексић, Мара; Рупар, Јелена; Брборић, Јасмина; Чудина, Оливера; | |
dc.citation.volume | 135 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000579730600005 | |
dc.identifier.doi | 10.1016/j.bioelechem.2020.107579 | |
dc.identifier.scopus | 2-s2.0-85086144465 | |
dc.type.version | publishedVersion | |