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Spectroscopic studies on the drug–drug interaction: the influence of fluoroquinolones on the affinity of tigecycline to human serum albumin and identification of the binding site

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2020
Authors
Stojanović, Stefan
Nićiforović, Jovan
Živanović, Sandra
Odović, Jadranka
Jelić, Ratomir
Article (Published version)
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Abstract
bstract: Concomitant use of two or more drugs in therapy is becoming a more frequent phenomenon and clinically relevant drug–drug interactions at the level of binding to human serum albumin (HSA) are more often. The influence of fluoroquinolones–sparfloxacin, levofloxacin, and ciprofloxacin, on the interaction between tigecycline and HSA, was investigated in vitro by means of fluorescence and absorption spectroscopy. The results of UV–Vis and fluorescence spectroscopy showed that the fluorescence quenching of HSA was a result of the formation of new complexes through a static quenching process. The binding constants (Ka) and the number of binding sites (n) of all systems were calculated. The presence of sparfloxacin and ciprofloxacin increases and that of levofloxacin slightly decreases the binding constant of the HSA–tigecycline system. Competitive binding studies in the presence of site-specific markers showed that tigecycline was not significantly displaced by ibuprofen, but warfari...n showed a significant displacement of tigecycline. These results suggest that the competitive binding of tigecycline and warfarin to HSA exists. The results confirm that the binding site of tigecycline is mainly located in Sudlow’s site I (subdomain IIA) of HSA. Graphic abstract: [Figure not available: see fulltext.

Keywords:
Absorption / Competitive binding / Fluorescence / Fluoroquinolones / Human serum albumin / Tigecycline
Source:
Monatshefte fur Chemie, 2020, 151, 999-1007
Publisher:
  • Springer
Projects:
  • Synthesis, modeling, physicochemical and biological properties of organic compounds and related metal complexes (RS-172016)
  • Pharmacological analysis of effects of biologically active substances on isolated smooth muscles from human gastrointestinal and urogenital tract (RS-175007)

DOI: 10.1007/s00706-020-02627-0

ISSN: 0026-9247

WoS: 000537679200001

Scopus: 2-s2.0-85085886775
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/3614
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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