Show simple item record

dc.creatorPirković-Čabarkapa, Andrea
dc.creatorŽivković, Lada
dc.creatorZlatković-Švenda, Mirjana
dc.creatorBorozan, Sunĉica
dc.creatorTopalović, Dijana
dc.creatorDekanski, Dragana
dc.creatorBruić, Marija
dc.creatorBajić, Vladan
dc.creatorRadak-Perović, Marija
dc.creatorSpremo-Potparević, Biljana
dc.date.accessioned2020-07-08T11:52:56Z
dc.date.available2020-07-08T11:52:56Z
dc.date.issued2020
dc.identifier.issn0534-0012
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
dc.description.abstractOxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.en
dc.publisherpublishedVersion
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.rightsopenAccess
dc.sourceGenetika
dc.subjectChromosome aberrations
dc.subjectDNA damage
dc.subjectDry olive leaf extract
dc.subjectMicronuclei
dc.subjectRheumatoid arthritis
dc.titleCytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extracen
dc.typearticle
dc.rights.licenseBY-NC-ND
dcterms.abstractЖивковић, Лада; Радак-Перовић, Марија; Спремо-Потпаревић, Биљана; Бајић, Владан; Пирковић-Чабаркапа, Aндреа; Златковић-Швенда, Мирјана; Борозан, Сунĉица; Бруић, Марија; Декански, Драгана; Топаловић, Дијана;
dc.citation.volume52
dc.citation.issue1
dc.citation.spage67
dc.citation.epage80
dc.citation.rankM23~
dc.identifier.wos000535652400006
dc.identifier.doi10.2298/GENSR2001067P
dc.identifier.scopus2-s2.0-85087124013
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/7865/CYTOGENETIC_ALTERATIONS_IN_PUB_2020.pdf
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record