Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots
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2020
Authors
Mudrić, JelenaJanković, Teodora
Šavikin, Katarina

Bigović, Dubravka
Đukić-Ćosić, Danijela

Ibrić, Svetlana

Đuriš, Jelena

Article (Published version)

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Perennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of... 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed.
Keywords:
Artificial neural networks (ANN) / Gentiopicrin / Heat-assisted extraction / Isogentisin / Response surface methodology (RSM) / Yellow gentianSource:
Industrial Crops and Products, 2020, 155Publisher:
- Elsevier B.V.
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200003 (Institute for Medicinal Plant Research 'Dr. Josif Pančić ', Belgrade) (RS-200003)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200161 (University of Belgrade, Faculty of Pharmacy) (RS-200161)
DOI: 10.1016/j.indcrop.2020.112767
ISSN: 0926-6690
WoS: 000571868400001
Scopus: 2-s2.0-85088026455
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PharmacyTY - JOUR AU - Mudrić, Jelena AU - Janković, Teodora AU - Šavikin, Katarina AU - Bigović, Dubravka AU - Đukić-Ćosić, Danijela AU - Ibrić, Svetlana AU - Đuriš, Jelena PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3636 AB - Perennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed. PB - Elsevier B.V. T2 - Industrial Crops and Products T1 - Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots VL - 155 DO - 10.1016/j.indcrop.2020.112767 ER -
@article{ author = "Mudrić, Jelena and Janković, Teodora and Šavikin, Katarina and Bigović, Dubravka and Đukić-Ćosić, Danijela and Ibrić, Svetlana and Đuriš, Jelena", year = "2020", abstract = "Perennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed.", publisher = "Elsevier B.V.", journal = "Industrial Crops and Products", title = "Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots", volume = "155", doi = "10.1016/j.indcrop.2020.112767" }
Mudrić, J., Janković, T., Šavikin, K., Bigović, D., Đukić-Ćosić, D., Ibrić, S.,& Đuriš, J.. (2020). Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots. in Industrial Crops and Products Elsevier B.V.., 155. https://doi.org/10.1016/j.indcrop.2020.112767
Mudrić J, Janković T, Šavikin K, Bigović D, Đukić-Ćosić D, Ibrić S, Đuriš J. Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots. in Industrial Crops and Products. 2020;155. doi:10.1016/j.indcrop.2020.112767 .
Mudrić, Jelena, Janković, Teodora, Šavikin, Katarina, Bigović, Dubravka, Đukić-Ćosić, Danijela, Ibrić, Svetlana, Đuriš, Jelena, "Optimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots" in Industrial Crops and Products, 155 (2020), https://doi.org/10.1016/j.indcrop.2020.112767 . .