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dc.creatorMudrić, Jelena
dc.creatorJanković, Teodora
dc.creatorŠavikin, Katarina
dc.creatorBigović, Dubravka
dc.creatorĐukić-Ćosić, Danijela
dc.creatorIbrić, Svetlana
dc.creatorĐuriš, Jelena
dc.date.accessioned2020-07-28T10:11:04Z
dc.date.available2020-07-28T10:11:04Z
dc.date.issued2020
dc.identifier.issn0926-6690
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/3636
dc.description.abstractPerennial plant Gentiana lutea L. is used worldwide for the preparation of pharmaceutical and food products. Health benefits of G. lutea roots are associated with the presence of major bitter-tasting secoiridoid gentiopicroside and xanthone isogentisin. The aim was to optimize the heat-assisted extraction of gentipicroside (GP), isogentisin (ISG) and total phenolics (TP) from G. lutea roots and develop models with high accuracy and prediction capacity by response surface methodology (RSM) and artificial neural networks (ANN). Extracts were prepared according to central composite design. Significant independent variables which were previously identified by Plackett-Burman screening design were varied at five levels - temperature (20−80 °C), time (8−180 min), solid-to-solvent ratio (1:10−1:50) and ethanol concentration (10–70 %). Contents of GP, ISG (by HPLC-DAD) and TP (by Folin–Ciocalteu method) were analyzed. The optimal conditions for the extraction were temperature of 65 °C, time of 129.08 min, solid-to-solvent ratio of 1:40, and ethanol concentration 49.33 %. Under these conditions, experimentally obtained results for GP (18.03 mg/g dw), ISG (8.15 mg/g dw) and TP (17.46 mg of gallic acid equivalents/g dw) content were in agreement with the values predicted by RSM and ANN. Comparison of models through the coefficient of determination (R2) and the root mean square error (RMSE) showed that ANN approach was superior to RSM in predicting and modelling GP, ISG and TP content, simultaneously. Effective heat-assisted extraction method for the extraction of GP, ISG and TP from the roots of G. lutea was designed, and models with high accuracy and good prediction capacity were developed.
dc.publisherElsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200003/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.relation
dc.rightsrestrictedAccess
dc.sourceIndustrial Crops and Products
dc.subjectArtificial neural networks (ANN)
dc.subjectGentiopicrin
dc.subjectHeat-assisted extraction
dc.subjectIsogentisin
dc.subjectResponse surface methodology (RSM)
dc.subjectYellow gentian
dc.titleOptimization and modelling of gentiopicroside, isogentisin and total phenolics extraction from Gentiana lutea L. roots
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЂуриш, Јелена; Ђукић-Ћосић, Данијела; Мудрић, Јелена; Јанковић, Теодора; Шавикин, Катарина; Биговић, Дубравка; Ибрић, Светлана;
dc.citation.volume155
dc.identifier.doi10.1016/j.indcrop.2020.112767
dc.identifier.scopus2-s2.0-85088026455
dc.type.versionpublishedVersion


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