Investigation of antihyperalgesic and antiedematous activities of three Hieracium species
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2020
Authors
Milutinović, Violeta
Pecikoza, Uroš

Tomić, Maja

Stepanović-Petrović, Radica

Niketić, Marjan
Ušjak, Ljuboš

Petrović, Silvana

Article (Published version)

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Show full item recordAbstract
Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of m...otor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.
Keywords:
Hieracium / triterpene/polyphenol rich extracts / carrageenan-induced inflammation / antihyperalgesic activity / antiedematous activity / acute toxicity screeningSource:
Natural Product Research, 2020Publisher:
- Taylor & Francis Ltd
Projects:
- Examination of mechanisms of action, toxicity and interactions of adjuvant analgesics (RS-175045)
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-173021)
DOI: 10.1080/14786419.2020.1768086
ISSN: 1478-6419
PubMed: 32441121