Investigation of antihyperalgesic and antiedematous activities of three Hieracium species
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2020
Authors
Milutinović, Violeta
Pecikoza, Uroš

Tomić, Maja

Stepanović-Petrović, Radica

Niketić, Marjan

Ušjak, Ljuboš

Petrović, Silvana

Article (Published version)

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Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of m...otor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.
Keywords:
Hieracium / triterpene/polyphenol rich extracts / carrageenan-induced inflammation / antihyperalgesic activity / antiedematous activity / acute toxicity screeningSource:
Natural Product Research, 2020Publisher:
- Taylor & Francis Ltd
Funding / projects:
- Examination of mechanisms of action, toxicity and interactions of adjuvant analgesics (RS-175045)
- Investigation on the medicinal plants: morphological, chemical and pharmacological characterisation (RS-173021)
DOI: 10.1080/14786419.2020.1768086
ISSN: 1478-6419
PubMed: 32441121
WoS: 000538733200001
Scopus: 2-s2.0-85085613046
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Institution/Community
PharmacyTY - JOUR AU - Milutinović, Violeta AU - Pecikoza, Uroš AU - Tomić, Maja AU - Stepanović-Petrović, Radica AU - Niketić, Marjan AU - Ušjak, Ljuboš AU - Petrović, Silvana PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3641 AB - Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity. PB - Taylor & Francis Ltd T2 - Natural Product Research T1 - Investigation of antihyperalgesic and antiedematous activities of three Hieracium species DO - 10.1080/14786419.2020.1768086 ER -
@article{ author = "Milutinović, Violeta and Pecikoza, Uroš and Tomić, Maja and Stepanović-Petrović, Radica and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana", year = "2020", abstract = "Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.", publisher = "Taylor & Francis Ltd", journal = "Natural Product Research", title = "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species", doi = "10.1080/14786419.2020.1768086" }
Milutinović, V., Pecikoza, U., Tomić, M., Stepanović-Petrović, R., Niketić, M., Ušjak, L.,& Petrović, S.. (2020). Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research Taylor & Francis Ltd.. https://doi.org/10.1080/14786419.2020.1768086
Milutinović V, Pecikoza U, Tomić M, Stepanović-Petrović R, Niketić M, Ušjak L, Petrović S. Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research. 2020;. doi:10.1080/14786419.2020.1768086 .
Milutinović, Violeta, Pecikoza, Uroš, Tomić, Maja , Stepanović-Petrović, Radica, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species" in Natural Product Research (2020), https://doi.org/10.1080/14786419.2020.1768086 . .