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Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Authorized Users Only
2020
Authors
Stevanović, Milena
Đošić, Marija
Janković, Ana
Kojić, Vesna
Vukašinović-Sekulić, Maja
Stojanović, Jovica
Odović, Jadranka
Crevar-Sakač, Milkica
Kyong Yop, Rhee
Mišković-Stanković, Vesna
Article (Published version)
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Abstract
Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition stron...gly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.

Keywords:
antibacterial activity / cytotoxicity / electrophoretic deposition / gentamicin / graphene
Source:
Journal of Biomedical Materials Research - Part A, 2020, 108, 11, 2175-2189
Publisher:
  • Wiley Periodicals, LLC.
Projects:
  • Synthesis, processing and applications of nanostructured multifunctional materials with defined properties (RS-45019)
  • Basic Science Research Program of the Ministryof Education, Science and Technology of Korea (GrantNo. 2018R1A2B5A02023190).

DOI: 10.1002/jbm.a.36974

ISSN: 1549-3296

WoS: 000543218000001

Scopus: 2-s2.0-85087206871
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URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/3657
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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