Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species
Authors
Ignjatović, Janko
Maljurić, Nevena

Golubović, Jelena

Ravnikar, Matjaž
Petković, Miloš

Savodnik, Nika
Štrukelj, Borut
Otašević, Biljana

Article (Published version)
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Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from conifer needles was selected as appropriately promising natural source for drug discovery. Its dichloromethane and ethanol extracts considerably inhibited growth of Escherichia coli and Staphylococcus aureus. Moreover, the indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with well-established drugs from the same therapeutic category, two dominant compounds (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-d...ihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-3) and (Z)-(Z)-2-acetoxyprop-1-en-1-yl 3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-5) were recognized as valuable leading structures for future discovery of novel antibiotics.
Keywords:
Antibacterial activity / Endophytic fungi / GC-MS / HPLC / NMRSource:
Acta Chimica Slovenica, 2020, 67, 2, 445-461Publisher:
- Slovenian Chemical Society
Funding / projects:
- Synthesis, Quantitative Structure and Activity Relationship, Physico-Chemical Characterisation and Analysis of Pharmacologically Active Substances (RS-172033)
- Slovenian Re-search Agency (grant number P4-0127)
- EU (project no. BI-RS/16-17-022).
DOI: 10.17344/acsi.2019.5389
ISSN: 1318-0207
WoS: 000541748400010
Scopus: 2-s2.0-85090516475
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Institution/Community
PharmacyTY - JOUR AU - Ignjatović, Janko AU - Maljurić, Nevena AU - Golubović, Jelena AU - Ravnikar, Matjaž AU - Petković, Miloš AU - Savodnik, Nika AU - Štrukelj, Borut AU - Otašević, Biljana PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3661 AB - Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from conifer needles was selected as appropriately promising natural source for drug discovery. Its dichloromethane and ethanol extracts considerably inhibited growth of Escherichia coli and Staphylococcus aureus. Moreover, the indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with well-established drugs from the same therapeutic category, two dominant compounds (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-3) and (Z)-(Z)-2-acetoxyprop-1-en-1-yl 3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-5) were recognized as valuable leading structures for future discovery of novel antibiotics. PB - Slovenian Chemical Society T2 - Acta Chimica Slovenica T1 - Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species VL - 67 IS - 2 SP - 445 EP - 461 DO - 10.17344/acsi.2019.5389 ER -
@article{ author = "Ignjatović, Janko and Maljurić, Nevena and Golubović, Jelena and Ravnikar, Matjaž and Petković, Miloš and Savodnik, Nika and Štrukelj, Borut and Otašević, Biljana", year = "2020", abstract = "Recently, growing interest is devoted to investigation of bioactive secondary metabolites of endophytic fungi. Thus, as an extension to our previous achievements related to antimicrobial potential of endophytic fungi, Phomopsis species isolated from conifer needles was selected as appropriately promising natural source for drug discovery. Its dichloromethane and ethanol extracts considerably inhibited growth of Escherichia coli and Staphylococcus aureus. Moreover, the indi-vidual compounds of dichloromethane extract have been separated, collected and purified using semi preparative liquid chromatographic analysis and comprehensively characterized using mass spectrometry (MS) and nuclear magnetic res-onance spectroscopy (NMR). Based on their antimicrobial activity and unique structural characteristics in comparison with well-established drugs from the same therapeutic category, two dominant compounds (Z)-(Z)-2-acetoxyprop-1-en-1-yl-3-(3-((E)-3,4-dihydroxypent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-3) and (Z)-(Z)-2-acetoxyprop-1-en-1-yl 3-(3-((E)-4-hydroxy-3-oxopent-1-en-1-yl)oxiran-2-yl)acrylate (denoted as 325-5) were recognized as valuable leading structures for future discovery of novel antibiotics.", publisher = "Slovenian Chemical Society", journal = "Acta Chimica Slovenica", title = "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species", volume = "67", number = "2", pages = "445-461", doi = "10.17344/acsi.2019.5389" }
Ignjatović, J., Maljurić, N., Golubović, J., Ravnikar, M., Petković, M., Savodnik, N., Štrukelj, B.,& Otašević, B.. (2020). Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica Slovenian Chemical Society., 67(2), 445-461. https://doi.org/10.17344/acsi.2019.5389
Ignjatović J, Maljurić N, Golubović J, Ravnikar M, Petković M, Savodnik N, Štrukelj B, Otašević B. Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species. in Acta Chimica Slovenica. 2020;67(2):445-461. doi:10.17344/acsi.2019.5389 .
Ignjatović, Janko, Maljurić, Nevena, Golubović, Jelena, Ravnikar, Matjaž, Petković, Miloš, Savodnik, Nika, Štrukelj, Borut, Otašević, Biljana, "Characterization of biomolecules with antibiotic activity from endophytic fungi phomopsis species" in Acta Chimica Slovenica, 67, no. 2 (2020):445-461, https://doi.org/10.17344/acsi.2019.5389 . .