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The significance of biochemical markers and morphometric characteristics of leucocytes in laboratory diagnosis of subclinical cobalamin deficiency

dc.contributor.otherMarisavljević, Dragomir
dc.contributor.otherMirković, Duško
dc.contributor.otherDopsaj, Violeta
dc.creatorTotošković, Dragana
dc.date.accessioned2020-10-13T09:51:15Z
dc.date.available2020-10-13T09:51:15Z
dc.date.issued2020
dc.identifier.urihttp://eteze.bg.ac.rs/application/showtheses?thesesId=7490
dc.identifier.urihttps://fedorabg.bg.ac.rs/fedora/get/o:22368/bdef:Content/download
dc.identifier.urihttp://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=15294473
dc.identifier.urihttp://nardus.mpn.gov.rs/handle/123456789/17294
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3705
dc.description.abstractSubklinički deficit vitamina B12 (engl. Subclinical Cobalamin Deficiency,SCCD) podrazumeva da je koncentracija jednog ili više biomarkera statusa vitaminaB12 izvan granica referentnog opsega u odsustvu kliničkih manifestacija deficita. Dosada se mali broj studija bavio ovim oblikom nedostatka kobalamina kod starijihpacijenata sa rizikom za deficit vitamina B12, a koji nemaju kliničku sliku deficita.Otkrivanje ranih promena u statusu vitamina B12 značajno je za ovu populaciju, jer seneurološki simptomi deficita mogu javiti u odsustvu hematoloških simptoma i mogu bitiireverzibilni, ukoliko se terapija ne primeni na vreme. Kako se SCCD potvrđujeisključivo na osnovu laboratorijskog testiranja statusa vitamina B12, značajno jeanalizirati odnos rutinskih i novih biomarkera (metilmalonske kiseline (MMA) imorfometrijskih parametara leukocita) u populaciji sa rizikom za pojavu deficitakobalamina.CILJEVI: Cilj studije jeste ispitivanje značaja biohemijskih markera i morfometrijskihkarakteristika leukocita u laboratorijskoj dijagnostici subkliničkog deficita vitaminaB12 u odsustvu kliničkih simptoma. Takođe, cilj rada je i utvrđivanje povezanostimetilmalonske kiseline sa postojećim parametrima za ispitivanje statusa vitamina B12 imorfometrijskim parametrima neutrofila i monocita, dobijenih na Coulter® LH750hematološkom analizatoru, kao potencijalno ranih markera promena u statusu vitaminaB12.MATERIJALI I METODE: Studija je obuhvatila 590 pacijenata sa rizikom za deficitkobalamina, kao i 148 zdravih dobrovoljaca. Pacijenti sa rizikom za deficit kobalaminabili su stariji od 60 godina i/ili osobe sa gastrointestinalnim smetnjama i simptomima.Za potrebe studije urađene su sledeće analize: krvna slika sa morfometrijskimparametrima leukocita, kobalamin, folat, folat u eritrocitima, antitela protiv unutrašnjegfaktora, homocistein (Hcy), serumsko gvožđe, solubilni transferinski receptori, feritin,transferin, ukupni kapacitet vezivanja gvožđa, saturacija transferina gvožđem,haptoglobin, laktat dehidrogenaza, kreatinin, procenjena brzina glomerularne filtracije...sr
dc.description.abstractIn the absence of clinical manifestations, concentration of one ormore biomarkers of vitamin B12 status out of reference range is considered assubclinical cobalamin deficiency (SCCD). So far, the small number of studies haveexplored SCCD in elderly patients at risk of vitamin B12 deficiency, but withoutclinical picture. The recognition of early changes in cobalamin status is important forthis population because neurological symptoms of deficiency can be present withouthematological manifestations. Also, neurological symptoms of deficiency can beirreversible without timely therapy. Considering that SCCD depends solely onlaboratory testing of vitamin B12 status, it is important to analyse relationship betweenbiomarkers routinely used and novel markers (methylmalonic acid (MMA) andmorphometric parameters of leucocytes) in the population at risk of cobalamindeficiency.AIMS OF STUDY: The study objective is the research of significance of biochemicalmarkers and morphometric parameters of leucocytes in laboratory diagnosis ofsubclinical cobalamin deficiency in the absence of clinical manifestations. Also, thestudy aim is to analyse relationships between methylmalonic acid and biomarkersroutinely used, and morphometric parameters of neutrophils and monocytes, obtainedby Coulter® LH750 hematology analyser, as potential early markers of changes invitamin B12 status.MATERIALS AND METHODS: The study population included 590 patients at riskof cobalamin deficiency and 148 healthy volunteers. The patients at risk of cobalamindeficiency were older than 60 years and /or had gastrointestinal symptoms or disorders.For the study purpose, the following tests were done: complete blood count withmorphometric parameters of leucocytes, cobalamin, folate, red blood cell folate,intrinsic factor antibody, homocysteine (Hcy), serum iron, soluble transferrin receptors,ferritin, transferrin, total iron binding-capacity, transferrin saturation, haptoglobin,lactate dehydrogenase, creatinine, estimated glomerular filtration rate...en
dc.formatapplication/pdf
dc.languagesr
dc.publisherУниверзитет у Београду, Фармацеутски факултетsr
dc.rightsopenAccess
dc.sourceУниверзитет у Београдуsr
dc.subjectsubklinički deficit kobalamina, vitamin B12, folat, metilmalonskakiselina, homocistein, morfometrijski parametri neutrofila i monocitasr
dc.subjectsubclinical cobalamin deficiency, vitamin B12, folate, methylmalonic acid,homocysteine, morphometric parameters of neutrophils and monocytesen
dc.titleZnačaj biohemijskih markera i morfometrijskih karakteristika leukocita u laboratorijskoj dijagnostici subkliničkog deficita vitamina B12sr
dc.title.alternativeThe significance of biochemical markers and morphometric characteristics of leucocytes in laboratory diagnosis of subclinical cobalamin deficiencyen
dc.typedoctoralThesisen
dc.rights.licenseBY-NC-ND
dcterms.abstractДопсај, Виолета; Мирковић, Душко; Марисављевић, Драгомир; Тотошковић, Драгана; Значај биохемијских маркера и морфометријских карактеристика леукоцита у лабораторијској дијагностици субклиничког дефицита витамина Б12; Значај биохемијских маркера и морфометријских карактеристика леукоцита у лабораторијској дијагностици субклиничког дефицита витамина Б12;
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/8310/IzvestajKomisije22931.pdf
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/8309/Disertacija.pdf
dc.type.versionpublishedVersion


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