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Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora

Nutritional, chemical and biological characterisation of seaweeds Halimeda tuna, Codium bursa, Cystoseira barbata and seagrass Cymodocea nodosa from the Adriatic Sea

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2020
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Authors
Milović, Sanja
Contributors
Stanković, Ivan
Kundaković-Vasović, Tatjana
Stanojković, Tatjana
Petović, Slavica
Doctoral thesis (Published version)
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Abstract
Predmet istraživanja ove doktorske disertacije su tri morske alge: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) i morskatrava Cymodocea nodosa (Urcia) Ascherson (Cymodoceaceae) iz Jadranskog mora.Nutritivna vrednost odabranih uzoraka je ispitivana korišćenjem zvaničnih AOAC(Association of Official Analytical Chemists) metoda. Najveću energetsku vrednost, unavedenim ispitivanjima, imala je zelena alga C. bursa (490,4 kcal).Metodom hladne maceracije u trajanju od 48 sati, dobijena su tri polarna ekstrakta:dihlormetan : metanolni (1:1) (DME), 70% metanolni i vodeni ekstrakt. Suvi ekstrakti su daljekorišćeni za biološka in vitro ispitivanja kao što su: antioksidativna, antimikrobna, anti α-glukozidazna i citotoksična aktivnost. DME ekstrakt H. tuna je pokazao najbolju citotoksičnuaktivnost prema ćelijama humanog karcinoma kolona, LS174 (IC50=17,92 ± 1,54 μg/ml).DME ekstr...akt C. nodosa je pokazao snažnu citotoksičnu aktivnost prema ćelijama humanogadenokarcinoma, HeLa (IC50=13,28 ± 0,39 μg/ml) i prema ćelijama humane hroničnemijelogene leukemije, K562 (IC50=19,64 ± 1,55 μg/ml). Najbolju anti α-glukozidaznuaktivnost (IC50=9,98 ± 3,34 μg/ml) i najbolju antibakterijsku aktivnost (MIC 100 μg/ml)prema Staphylococcus aureus i Bacillus subtilis je ispoljio DME ekstrakt C. barbata.Razvojom odgovarajuće HPLC metode, kao i primenom odgovarajućih metodadetekcije identifikovana su tri jedinjenja (diosmetin-7-sulfat, kaftarinska i kutarinska kiselina)u DME ekstraktu morske trave C. nodosa.Sadržaj β-glukana, masnih kiselina, sterola i mikro- i makroelemenata je ispitivan usvim navedenim uzorcima. Najveći sadržaj β-glukana bio je u morskoj travi C. nodosa (13,04± 0,42 g/100 g). C. nodosa, morska trava, pokazala je najviši sadržaj polinezasićenih masnihkiselina (PUFA, 7,157 mg/g). Značajan sadržaj sterola (fukosterol, 21,76 ± 0,1 μg/g) i najboljiodnos polinezasićenih i zasićenih (SFA) masnih kiselina, PUFA/SFA (0,08) pokazan je kodmrke alge C. barbata. Zelene alge, C. bursa i H. tuna, imale su najveći saržaj sterola (β-sitosterol, 95,21 ± 0,16 μg/g i 73,90 ± 0,08 μg/g, respektivno). Najviši sadržaj kalcijuma (Ca),u količini od 55125 μg/g, detektovan je kod alge H. tuna.

The research subject of doctoral dissertation were three seaweeds: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) and the oneseagrass Cymodocea nodosa (Urcia) Acherson (Cymodoceaceae) from the Adriatic Sea.Nutritional value of selected samples were investigated by official AOAC(Association of Official Analytical Chemists) methods. The highest energy value had thegreen seaweed C. bursa (490,4 kcal).Using the cold maceration extraction, lasting 48 h, the three polar extracts wereobtained: dichlormetan: methanol (1:1) (DME), 70 % methanol and aquous extracts. The dryextracts were further used for biological in vitro investigations, such as: antioxidative,antimicrobial, anti α-glucosidase and cytotoxic activity.DME extract of H. tuna has shown the best cytotoxic activity against human coloncarcinoma cell line, LS174 (IC50=17,92 ± 1,54 μg/ml). DME extract of C. nodosa had s...trongcytotoxicity against human adenocarcinoma cell line, HeLa (IC50=13,28 ± 0,39 μg/ml) andhuman chronic myelogenous leukaemia cell line, K562 (IC50=19,64 ± 1,55 μg/ml). The bestanti α-glucosidase (IC50=9,98 ± 3,34 μg/ml) and best antibacterial activity (minimal inhibitoryconcentration of 100 μg/ml) for Staphylococcus aureus and Bacillus subtilis wasdemonstrated in DME extract of seagrass C. nodosa.Developing the appropriate HPLC method, accompained with the appropriatedetection methods, the three compounds were identified (diosmetin-7-sulfate, caftaric andcoutaric acid) in the DME extract of seagrass C. nodosa.The content of β-glucan, fatty acids, sterols and micro- and macroelements wereinvestigated in all samples. The highest content of β-glucan was detected in C. nodosaseagrass (13,04 ± 0,42 g/100 g). C. nodosa had also the highest level of polyunsaturated fattyacids (PUFAs) (7,157 mg/g). Significant sterol content (fucosterol, 21,76 ± 0,1 μg/g) and thebest ratio of polyunsaturated and saturated (SFA) fatty acids, PUFA/SFA (0,08) was shown inC. barbata, brown seaweed. Green algae, C. bursa and H. tuna, showed the highest level ofsterols (β-sitosterol, 95,21 ± 0,16 μg/g and 73,90 ± 0,08 μg/g, respectively). H. tuna had thehighest content of calcium (Ca) in amount of 55125 μg/g.

Keywords:
Halimeda tuna / Halimeda tuna / Codium bursa / Cystoseira barbata / Cymodocea nodosa / seaweeds / seagrass / Codium bursa / Cystoseira barbata / Cymodocea nodosa / alge / morska trava
Source:
Универзитет у Београду, 2020
Publisher:
  • Универзитет у Београду, Фармацеутски факултет
[ Google Scholar ]
Handle
https://hdl.handle.net/21.15107/rcub_nardus_17520
URI
http://eteze.bg.ac.rs/application/showtheses?thesesId=7652
https://fedorabg.bg.ac.rs/fedora/get/o:22807/bdef:Content/download
http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23145225
https://nardus.mpn.gov.rs/handle/123456789/17520
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3722
Collections
  • Doktorati
Institution/Community
Pharmacy
TY  - THES
AU  - Milović, Sanja
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7652
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:22807/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23145225
UR  - https://nardus.mpn.gov.rs/handle/123456789/17520
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3722
AB  - Predmet istraživanja ove doktorske disertacije su tri morske alge: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) i morskatrava Cymodocea nodosa (Urcia) Ascherson (Cymodoceaceae) iz Jadranskog mora.Nutritivna vrednost odabranih uzoraka je ispitivana korišćenjem zvaničnih AOAC(Association of Official Analytical Chemists) metoda. Najveću energetsku vrednost, unavedenim ispitivanjima, imala je zelena alga C. bursa (490,4 kcal).Metodom hladne maceracije u trajanju od 48 sati, dobijena su tri polarna ekstrakta:dihlormetan : metanolni (1:1) (DME), 70% metanolni i vodeni ekstrakt. Suvi ekstrakti su daljekorišćeni za biološka in vitro ispitivanja kao što su: antioksidativna, antimikrobna, anti α-glukozidazna i citotoksična aktivnost. DME ekstrakt H. tuna je pokazao najbolju citotoksičnuaktivnost prema ćelijama humanog karcinoma kolona, LS174 (IC50=17,92 ± 1,54 μg/ml).DME ekstrakt C. nodosa je pokazao snažnu citotoksičnu aktivnost prema ćelijama humanogadenokarcinoma, HeLa (IC50=13,28 ± 0,39 μg/ml) i prema ćelijama humane hroničnemijelogene leukemije, K562 (IC50=19,64 ± 1,55 μg/ml). Najbolju anti α-glukozidaznuaktivnost (IC50=9,98 ± 3,34 μg/ml) i najbolju antibakterijsku aktivnost (MIC 100 μg/ml)prema Staphylococcus aureus i Bacillus subtilis je ispoljio DME ekstrakt C. barbata.Razvojom odgovarajuće HPLC metode, kao i primenom odgovarajućih metodadetekcije identifikovana su tri jedinjenja (diosmetin-7-sulfat, kaftarinska i kutarinska kiselina)u DME ekstraktu morske trave C. nodosa.Sadržaj β-glukana, masnih kiselina, sterola i mikro- i makroelemenata je ispitivan usvim navedenim uzorcima. Najveći sadržaj β-glukana bio je u morskoj travi C. nodosa (13,04± 0,42 g/100 g). C. nodosa, morska trava, pokazala je najviši sadržaj polinezasićenih masnihkiselina (PUFA, 7,157 mg/g). Značajan sadržaj sterola (fukosterol, 21,76 ± 0,1 μg/g) i najboljiodnos polinezasićenih i zasićenih (SFA) masnih kiselina, PUFA/SFA (0,08) pokazan je kodmrke alge C. barbata. Zelene alge, C. bursa i H. tuna, imale su najveći saržaj sterola (β-sitosterol, 95,21 ± 0,16 μg/g i 73,90 ± 0,08 μg/g, respektivno). Najviši sadržaj kalcijuma (Ca),u količini od 55125 μg/g, detektovan je kod alge H. tuna.
AB  - The research subject of doctoral dissertation were three seaweeds: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) and the oneseagrass Cymodocea nodosa (Urcia) Acherson (Cymodoceaceae) from the Adriatic Sea.Nutritional value of selected samples were investigated by official AOAC(Association of Official Analytical Chemists) methods. The highest energy value had thegreen seaweed C. bursa (490,4 kcal).Using the cold maceration extraction, lasting 48 h, the three polar extracts wereobtained: dichlormetan: methanol (1:1) (DME), 70 % methanol and aquous extracts. The dryextracts were further used for biological in vitro investigations, such as: antioxidative,antimicrobial, anti α-glucosidase and cytotoxic activity.DME extract of H. tuna has shown the best cytotoxic activity against human coloncarcinoma cell line, LS174 (IC50=17,92 ± 1,54 μg/ml). DME extract of C. nodosa had strongcytotoxicity against human adenocarcinoma cell line, HeLa (IC50=13,28 ± 0,39 μg/ml) andhuman chronic myelogenous leukaemia cell line, K562 (IC50=19,64 ± 1,55 μg/ml). The bestanti α-glucosidase (IC50=9,98 ± 3,34 μg/ml) and best antibacterial activity (minimal inhibitoryconcentration of 100 μg/ml) for Staphylococcus aureus and Bacillus subtilis wasdemonstrated in DME extract of seagrass C. nodosa.Developing the appropriate HPLC method, accompained with the appropriatedetection methods, the three compounds were identified (diosmetin-7-sulfate, caftaric andcoutaric acid) in the DME extract of seagrass C. nodosa.The content of β-glucan, fatty acids, sterols and micro- and macroelements wereinvestigated in all samples. The highest content of β-glucan was detected in C. nodosaseagrass (13,04 ± 0,42 g/100 g). C. nodosa had also the highest level of polyunsaturated fattyacids (PUFAs) (7,157 mg/g). Significant sterol content (fucosterol, 21,76 ± 0,1 μg/g) and thebest ratio of polyunsaturated and saturated (SFA) fatty acids, PUFA/SFA (0,08) was shown inC. barbata, brown seaweed. Green algae, C. bursa and H. tuna, showed the highest level ofsterols (β-sitosterol, 95,21 ± 0,16 μg/g and 73,90 ± 0,08 μg/g, respectively). H. tuna had thehighest content of calcium (Ca) in amount of 55125 μg/g.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17520
ER  - 
@phdthesis{
author = "Milović, Sanja",
year = "2020",
abstract = "Predmet istraživanja ove doktorske disertacije su tri morske alge: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) i morskatrava Cymodocea nodosa (Urcia) Ascherson (Cymodoceaceae) iz Jadranskog mora.Nutritivna vrednost odabranih uzoraka je ispitivana korišćenjem zvaničnih AOAC(Association of Official Analytical Chemists) metoda. Najveću energetsku vrednost, unavedenim ispitivanjima, imala je zelena alga C. bursa (490,4 kcal).Metodom hladne maceracije u trajanju od 48 sati, dobijena su tri polarna ekstrakta:dihlormetan : metanolni (1:1) (DME), 70% metanolni i vodeni ekstrakt. Suvi ekstrakti su daljekorišćeni za biološka in vitro ispitivanja kao što su: antioksidativna, antimikrobna, anti α-glukozidazna i citotoksična aktivnost. DME ekstrakt H. tuna je pokazao najbolju citotoksičnuaktivnost prema ćelijama humanog karcinoma kolona, LS174 (IC50=17,92 ± 1,54 μg/ml).DME ekstrakt C. nodosa je pokazao snažnu citotoksičnu aktivnost prema ćelijama humanogadenokarcinoma, HeLa (IC50=13,28 ± 0,39 μg/ml) i prema ćelijama humane hroničnemijelogene leukemije, K562 (IC50=19,64 ± 1,55 μg/ml). Najbolju anti α-glukozidaznuaktivnost (IC50=9,98 ± 3,34 μg/ml) i najbolju antibakterijsku aktivnost (MIC 100 μg/ml)prema Staphylococcus aureus i Bacillus subtilis je ispoljio DME ekstrakt C. barbata.Razvojom odgovarajuće HPLC metode, kao i primenom odgovarajućih metodadetekcije identifikovana su tri jedinjenja (diosmetin-7-sulfat, kaftarinska i kutarinska kiselina)u DME ekstraktu morske trave C. nodosa.Sadržaj β-glukana, masnih kiselina, sterola i mikro- i makroelemenata je ispitivan usvim navedenim uzorcima. Najveći sadržaj β-glukana bio je u morskoj travi C. nodosa (13,04± 0,42 g/100 g). C. nodosa, morska trava, pokazala je najviši sadržaj polinezasićenih masnihkiselina (PUFA, 7,157 mg/g). Značajan sadržaj sterola (fukosterol, 21,76 ± 0,1 μg/g) i najboljiodnos polinezasićenih i zasićenih (SFA) masnih kiselina, PUFA/SFA (0,08) pokazan je kodmrke alge C. barbata. Zelene alge, C. bursa i H. tuna, imale su najveći saržaj sterola (β-sitosterol, 95,21 ± 0,16 μg/g i 73,90 ± 0,08 μg/g, respektivno). Najviši sadržaj kalcijuma (Ca),u količini od 55125 μg/g, detektovan je kod alge H. tuna., The research subject of doctoral dissertation were three seaweeds: Halimeda tuna (J.Ellis & Solander) J.V. Lamouroux (Halimedaceae), Codium bursa (Olivi) C. Agardh(Codiaceae), Cystoseira barbata (Stackhouse) C. Agardh (Sargassaceae) and the oneseagrass Cymodocea nodosa (Urcia) Acherson (Cymodoceaceae) from the Adriatic Sea.Nutritional value of selected samples were investigated by official AOAC(Association of Official Analytical Chemists) methods. The highest energy value had thegreen seaweed C. bursa (490,4 kcal).Using the cold maceration extraction, lasting 48 h, the three polar extracts wereobtained: dichlormetan: methanol (1:1) (DME), 70 % methanol and aquous extracts. The dryextracts were further used for biological in vitro investigations, such as: antioxidative,antimicrobial, anti α-glucosidase and cytotoxic activity.DME extract of H. tuna has shown the best cytotoxic activity against human coloncarcinoma cell line, LS174 (IC50=17,92 ± 1,54 μg/ml). DME extract of C. nodosa had strongcytotoxicity against human adenocarcinoma cell line, HeLa (IC50=13,28 ± 0,39 μg/ml) andhuman chronic myelogenous leukaemia cell line, K562 (IC50=19,64 ± 1,55 μg/ml). The bestanti α-glucosidase (IC50=9,98 ± 3,34 μg/ml) and best antibacterial activity (minimal inhibitoryconcentration of 100 μg/ml) for Staphylococcus aureus and Bacillus subtilis wasdemonstrated in DME extract of seagrass C. nodosa.Developing the appropriate HPLC method, accompained with the appropriatedetection methods, the three compounds were identified (diosmetin-7-sulfate, caftaric andcoutaric acid) in the DME extract of seagrass C. nodosa.The content of β-glucan, fatty acids, sterols and micro- and macroelements wereinvestigated in all samples. The highest content of β-glucan was detected in C. nodosaseagrass (13,04 ± 0,42 g/100 g). C. nodosa had also the highest level of polyunsaturated fattyacids (PUFAs) (7,157 mg/g). Significant sterol content (fucosterol, 21,76 ± 0,1 μg/g) and thebest ratio of polyunsaturated and saturated (SFA) fatty acids, PUFA/SFA (0,08) was shown inC. barbata, brown seaweed. Green algae, C. bursa and H. tuna, showed the highest level ofsterols (β-sitosterol, 95,21 ± 0,16 μg/g and 73,90 ± 0,08 μg/g, respectively). H. tuna had thehighest content of calcium (Ca) in amount of 55125 μg/g.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17520"
}
Milović, S.. (2020). Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_17520
Milović S. Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora. in Универзитет у Београду. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_17520 .
Milović, Sanja, "Nutritivna, hemijska i biološka karakterizacija morskih algi Halimeda tuna, Codium bursa, Cystoseira barbata i morske trave Cymodocea nodosa iz Jadranskog mora" in Универзитет у Београду (2020),
https://hdl.handle.net/21.15107/rcub_nardus_17520 .

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