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dc.creatorĐajić, Nevena
dc.creatorOtašević, Biljana
dc.creatorMalenović, Anđelija
dc.creatorZečević, Mira
dc.creatorHolzgrabe, Ulrike
dc.creatorProtić, Ana
dc.date.accessioned2020-11-10T12:13:37Z
dc.date.available2020-11-10T12:13:37Z
dc.date.issued2021
dc.identifier.issn0731-7085
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3728
dc.description.abstractBinding between cyclodextrin (CD) cavity and guest molecule in Reversed Phase High-Performance Liquid Chromatography (RP-HPLC) is dynamic process. In general, increasing CD concentration is inducing inclusion complex formation, leading to reduction of analyte's retention time. Consequently, the shortness in retention time is a measure of complex stability in HPLC. However, under certain experimental conditions, the retention of some analytes could be prolonged even when concentration of CD in the mobile phase is increased. In order to reveal the cause of this unexpected retention behavior, the present study was carried on. The model mixture consisted of risperidone, olanzapine and their related impurities, while β-CD was selected among CDs, as in the previous study. In order to achieve fast equilibrium between free analyte and β-CD-analyte complex, β-CD was not added to the mobile phase, but only to the sample. Detection was performed with Corona Charged Aerosol Detector (CAD), suitable for non-chromophoric β-CD. When analyzing olanzapine impurity B-β-CD sample, three peaks were detected, namely free β-CD, β-CD-analyte complex and free analyte. The complex stability constant was calculated employing a modification of the Benesi-Hildebrandt equation and CAD has proven to be useful in complex stability constants assessment if retention of free analyte and β-CD-analyte complex is distinguished. For all other analytes only two peaks could be detected, because free analyte and formed complex are eluting at the same retention time. Under such circumstances, the authors proposed the methodology for calculating stability constants and confirmed its applicability to studied model mixture.en
dc.publisherElsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Pharmaceutical and Biomedical Analysis
dc.subjectHPLC
dc.subjectβ-Cyclodextrin
dc.subjectCAD
dc.subjectInclusion complexes
dc.subjectStability constants
dc.titleCorona Charged Aerosol Detector in studying retention and β-cyclodextrin complex stability using RP-HPLCen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Протић, Aна; Зечевић, Мира; Холзграбе, Улрике; Ђајић, Невена; Оташевић, Биљана;
dc.citation.volume193
dc.citation.rankM22~
dc.identifier.wos000600773600017
dc.identifier.doi10.1016/j.jpba.2020.113711
dc.identifier.scopus2-s2.0-85094938167
dc.type.versionpublishedVersion


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