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Mahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro

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2020
Mahonia_aquifolium_Extracts_pub_2020.pdf (1.682Mb)
Authors
Damjanović, Ana
Kolundžija, Branka
Matić, Ivana
Krivokuća, Ana
Zdunić, Gordana
Šavikin, Katarina
Janković, Radmila
Antić-Stanković, Jelena
Stanojković, Tatjana
Article (Published version)
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Abstract
Mahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials tha...n untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.

Keywords:
cytotoxicity / doxorubicin / human lung adenocarcinoma / Mahonia aquifolium / matrix metalloproteinases
Source:
Molecules (Basel, Switzerland), 2020, 25, 22
Publisher:
  • MDPI
Projects:
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200026 (University of Belgrade, Institute of Chemistry, Technology and Metallurgy - IChTM) (RS-200026)
  • Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 451-03-68/2020-14/200043 (Institute of Oncology and Radiology of Serbia, Belgrade) (RS-200043)

DOI: 10.3390/molecules25225233

ISSN: 1420-3049

WoS: 000594939500001

Scopus: 2-s2.0-85096082601
[ Google Scholar ]
URI
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3738
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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