Show simple item record

dc.creatorDamjanović, Ana
dc.creatorKolundžija, Branka
dc.creatorMatić, Ivana
dc.creatorKrivokuća, Ana
dc.creatorZdunić, Gordana
dc.creatorŠavikin, Katarina
dc.creatorJanković, Radmila
dc.creatorAntić-Stanković, Jelena
dc.creatorStanojković, Tatjana
dc.date.accessioned2020-11-24T09:03:33Z
dc.date.available2020-11-24T09:03:33Z
dc.date.issued2020
dc.identifier.issn1420-3049
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3738
dc.description.abstractMahonia aquifolium and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell-cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, M. aquifolium extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in MMP9 expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic M. aquifolium extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.en
dc.publisherMDPI
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200026/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200043/RS//
dc.rightsopenAccess
dc.sourceMolecules (Basel, Switzerland)
dc.subjectcytotoxicity
dc.subjectdoxorubicin
dc.subjecthuman lung adenocarcinoma
dc.subjectMahonia aquifolium
dc.subjectmatrix metalloproteinases
dc.titleMahonia aquifolium Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitroen
dc.typearticle
dc.rights.licenseBY
dcterms.abstractКривокућа, Aна; Јанковић, Радмила; Aнтић-Станковић, Јелена; Станојковић, Татјана; Дамјановић, Aна; Шавикин, Катарина; Колунджија, Бранка; Здунић, Гордана; Матић, Ивана;
dc.citation.volume25
dc.citation.issue22
dc.citation.rankM22
dc.identifier.wos000594939500001
dc.identifier.doi10.3390/molecules25225233
dc.identifier.scopus2-s2.0-85096082601
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/8446/Mahonia_aquifolium_Extracts_pub_2020.pdf
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record