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dc.creatorUšjak, Dušan
dc.creatorDinić, Miroslav
dc.creatorNovović, Katarina
dc.creatorIvković, Branka
dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.creatorMilenković, Marina
dc.date.accessioned2020-12-15T12:49:21Z
dc.date.available2020-12-15T12:49:21Z
dc.date.issued2021
dc.identifier.issn1612-1872
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3752
dc.description.abstractAn increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.en
dc.publisherWiley-VCH Verlag
dc.rightsrestrictedAccess
dc.sourceChemistry and Biodiversity
dc.subjectGene expression
dc.subjectAcinetobacter baumannii
dc.subjectchalcones
dc.subjectpolymerase chain reaction
dc.subjectvirulence factors
dc.titleMethoxy-Substituted Hydroxychalcone Reduces Biofilm Production, Adhesion and Surface Motility of Acinetobacter baumannii by Inhibiting ompA Gene Expressionen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИвковић, Бранка; Динић, Мирослав; Нововић, Катарина; Филиповић, Ненад; Стевановић, Магдалена; Ушјак, Душан; Миленковић, Марина;
dc.citation.volume18
dc.citation.issue1
dc.citation.rankM22~
dc.description.otherConference poster: [https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758]
dc.identifier.wos000596697700001
dc.identifier.doi10.1002/cbdv.202000786
dc.identifier.scopus2-s2.0-85097239451
dc.type.versionpublishedVersion


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