The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single... nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
TY - JOUR
AU - Jovčić, Branko
AU - Novović, Katarina
AU - Filipić, Brankica
AU - Velhner, Maja
AU - Todorović, Dalibor
AU - Matović, Kazimir
AU - Rašić, Zoran
AU - Nikolić, Sonja
AU - Kiškarolj, Ferenc
AU - Kojić, Milan
PY - 2020
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3760
AB - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
PB - MDPI AG
T2 - Antibiotics
T1 - Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia
VL - 9
IS - 12
SP - 1
EP - 13
DO - 10.3390/antibiotics9120886
ER -
@article{
author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rašić, Zoran and Nikolić, Sonja and Kiškarolj, Ferenc and Kojić, Milan",
year = "2020",
url = "https://farfar.pharmacy.bg.ac.rs/handle/123456789/3760",
abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.",
publisher = "MDPI AG",
journal = "Antibiotics",
title = "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia",
volume = "9",
number = "12",
pages = "1-13",
doi = "10.3390/antibiotics9120886"
}
Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rašić Z, Nikolić S, Kiškarolj F, Kojić M. Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. Antibiotics. 2020;9(12):1-13
Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rašić, Z., Nikolić, S., Kiškarolj, F.,& Kojić, M. (2020). Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia.
AntibioticsMDPI AG., 9(12), 1-13.
https://doi.org/10.3390/antibiotics9120886
Jovčić Branko, Novović Katarina, Filipić Brankica, Velhner Maja, Todorović Dalibor, Matović Kazimir, Rašić Zoran, Nikolić Sonja, Kiškarolj Ferenc, Kojić Milan, "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia" 9, no. 12 (2020):1-13,
https://doi.org/10.3390/antibiotics9120886 .
