Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia
Authors
Jovčić, BrankoNovović, Katarina

Filipić, Brankica

Velhner, Maja
Todorović, Dalibor
Matović, Kazimir
Rašić, Zoran
Nikolić, Sonja
Kiškarolj, Ferenc
Kojić, Milan

Article (Published version)
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The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single... nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.
Keywords:
Colistin / PmrB / Quinolones / Resistance / Salmonella / WGSSource:
Antibiotics, 2020, 9, 12, 1-13Publisher:
- MDPI AG
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200031 (Scientific Institute of Veterinary Medicine 'Novi Sad', Novi Sad) (RS-200031)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200042 (University of Belgrade, Institute of Molecular Genetics and Genetic Engineering) (RS-200042)
DOI: 10.3390/antibiotics9120886
ISSN: 2079-6382
WoS: 000601714000001
Scopus: 2-s2.0-85097565355
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PharmacyTY - JOUR AU - Jovčić, Branko AU - Novović, Katarina AU - Filipić, Brankica AU - Velhner, Maja AU - Todorović, Dalibor AU - Matović, Kazimir AU - Rašić, Zoran AU - Nikolić, Sonja AU - Kiškarolj, Ferenc AU - Kojić, Milan PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3760 AB - The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia. PB - MDPI AG T2 - Antibiotics T1 - Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia VL - 9 IS - 12 SP - 1 EP - 13 DO - 10.3390/antibiotics9120886 ER -
@article{ author = "Jovčić, Branko and Novović, Katarina and Filipić, Brankica and Velhner, Maja and Todorović, Dalibor and Matović, Kazimir and Rašić, Zoran and Nikolić, Sonja and Kiškarolj, Ferenc and Kojić, Milan", year = "2020", abstract = "The antimicrobial susceptibility testing was conducted on 174 single isolates from poultry farms in Serbia and it was determined that seven Salmonella spp. were multidrug resistant. Sixteen serotypes were detected, but only serotype Infantis confirmed reduced susceptibility to colistin. Seven colistin resistant Salmonella Infantis were studied in detail using the WGS approach. Three sequence types were identified corresponding to different epizootiology region. The isolate from the Province of Vojvodina 3842 and isolates from Jagodina (92 and 821) are represented by the sequence type ST413 and ST11, respectively. Four isolates from Kraljevo are ST32, a common S. Infantis sequence type in humans, poultry and food. The fosfomycin resistance gene fosA7 in isolate 3842 and the vgaA gene in isolate 8418/2948 encoding resistance to pleuromutilins were reported for the first time in serovar Infantis. The changes in relative expression of the phoP/Q, mgrB and pmrA/B genes were detected. Single nucleotide polymorphisms of the pmrB gene, including transitions Val164Gly or Val164Met, and Arg92Pro are described. Analyses of quinolone resistance determining region revealed substitutions Ser83Tyr in GyrA protein and Thr57Ser and Ser80Arg in ParC protein. Based on WGS data, there are two major clusters among analyzed Salmonella Infantis isolates from central Serbia.", publisher = "MDPI AG", journal = "Antibiotics", title = "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia", volume = "9", number = "12", pages = "1-13", doi = "10.3390/antibiotics9120886" }
Jovčić, B., Novović, K., Filipić, B., Velhner, M., Todorović, D., Matović, K., Rašić, Z., Nikolić, S., Kiškarolj, F.,& Kojić, M.. (2020). Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics MDPI AG., 9(12), 1-13. https://doi.org/10.3390/antibiotics9120886
Jovčić B, Novović K, Filipić B, Velhner M, Todorović D, Matović K, Rašić Z, Nikolić S, Kiškarolj F, Kojić M. Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia. in Antibiotics. 2020;9(12):1-13. doi:10.3390/antibiotics9120886 .
Jovčić, Branko, Novović, Katarina, Filipić, Brankica, Velhner, Maja, Todorović, Dalibor, Matović, Kazimir, Rašić, Zoran, Nikolić, Sonja, Kiškarolj, Ferenc, Kojić, Milan, "Genomic characteristics of colistin-resistant salmonella enterica subsp. Enterica serovar infantis from poultry farms in the Republic of Serbia" in Antibiotics, 9, no. 12 (2020):1-13, https://doi.org/10.3390/antibiotics9120886 . .