Genotoxicity of fluoride subacute exposure in rats and selenium intervention
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2021
Authors
Radovanović, JelenaAntonijević, Biljana

Kolarević, Stoimir
Milutinović-Smiljanić, Sanja
Mandić, Jelena
Vuković-Gačić, Branka
Bulat, Zorica

Ćurčić, Marijana

Kračun-Kolarević, Margareta
Sunjog, Karolina
Kostić-Vuković, Jovana
Jovanović Marić, Jovana
Antonijević-Miljaković, Evica

Đukić-Ćosić, Danijela

Buha-Đorđević, Aleksandra

Javorac, Dragana

Baralić, Katarina

Mandinić, Zoran
Article (Published version)

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The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver ...tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.
Keywords:
DNA damage / Comet assay / NaF / Subacute toxicity / Wistar ratsSource:
Chemosphere, 2021, 266Publisher:
- Elsevier Ltd
Funding / projects:
DOI: 10.1016/j.chemosphere.2020.128978
ISSN: 0045-6535
WoS: 000674624300047
Scopus: 2-s2.0-85097468208
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PharmacyTY - JOUR AU - Radovanović, Jelena AU - Antonijević, Biljana AU - Kolarević, Stoimir AU - Milutinović-Smiljanić, Sanja AU - Mandić, Jelena AU - Vuković-Gačić, Branka AU - Bulat, Zorica AU - Ćurčić, Marijana AU - Kračun-Kolarević, Margareta AU - Sunjog, Karolina AU - Kostić-Vuković, Jovana AU - Jovanović Marić, Jovana AU - Antonijević-Miljaković, Evica AU - Đukić-Ćosić, Danijela AU - Buha-Đorđević, Aleksandra AU - Javorac, Dragana AU - Baralić, Katarina AU - Mandinić, Zoran PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3761 AB - The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain. PB - Elsevier Ltd T2 - Chemosphere T1 - Genotoxicity of fluoride subacute exposure in rats and selenium intervention VL - 266 DO - 10.1016/j.chemosphere.2020.128978 ER -
@article{ author = "Radovanović, Jelena and Antonijević, Biljana and Kolarević, Stoimir and Milutinović-Smiljanić, Sanja and Mandić, Jelena and Vuković-Gačić, Branka and Bulat, Zorica and Ćurčić, Marijana and Kračun-Kolarević, Margareta and Sunjog, Karolina and Kostić-Vuković, Jovana and Jovanović Marić, Jovana and Antonijević-Miljaković, Evica and Đukić-Ćosić, Danijela and Buha-Đorđević, Aleksandra and Javorac, Dragana and Baralić, Katarina and Mandinić, Zoran", year = "2021", abstract = "The aims of this study were to: (i) examine the toxic effects of sodium fluoride (NaF) in blood, liver, spleen, and brain cells of Wistar rats after the subacute exposure; (ii) explore the potential protective properties of selenium (Se) against fluoride toxicity after the simultaneous administration. Twenty male Wistar rats, eight weeks old, weighing approximately 140–190 g, were divided into four experimental groups (n = 5) as follows: I control-tap water; II NaF 150 ppm; III NaF 150 ppm and Se 1.5 mg/L; IV Se 1.5 mg/L, and had available water with solutions ad libitum for 28 days. DNA damage detected by comet assay was confirmed in the liver, spleen, and brain cells, but not in blood. Selenium supplementation together with NaF decreased DNA damage in liver and spleen cells. According to the histological findings, no changes were observed in spleen and brain tissues after NaF administration. Unlike the observed Se protective effect on the DNA level, no significant reduction of liver tissue injury was observed after the NaF and Se treatment, resulting in mild inflammation. Data of this study suggest that DNA damage after NaF subacute exposure at moderately high concentration was reduced in liver and spleen cells due to Se supplementation, but a similar change was not seen in the brain.", publisher = "Elsevier Ltd", journal = "Chemosphere", title = "Genotoxicity of fluoride subacute exposure in rats and selenium intervention", volume = "266", doi = "10.1016/j.chemosphere.2020.128978" }
Radovanović, J., Antonijević, B., Kolarević, S., Milutinović-Smiljanić, S., Mandić, J., Vuković-Gačić, B., Bulat, Z., Ćurčić, M., Kračun-Kolarević, M., Sunjog, K., Kostić-Vuković, J., Jovanović Marić, J., Antonijević-Miljaković, E., Đukić-Ćosić, D., Buha-Đorđević, A., Javorac, D., Baralić, K.,& Mandinić, Z.. (2021). Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere Elsevier Ltd., 266. https://doi.org/10.1016/j.chemosphere.2020.128978
Radovanović J, Antonijević B, Kolarević S, Milutinović-Smiljanić S, Mandić J, Vuković-Gačić B, Bulat Z, Ćurčić M, Kračun-Kolarević M, Sunjog K, Kostić-Vuković J, Jovanović Marić J, Antonijević-Miljaković E, Đukić-Ćosić D, Buha-Đorđević A, Javorac D, Baralić K, Mandinić Z. Genotoxicity of fluoride subacute exposure in rats and selenium intervention. in Chemosphere. 2021;266. doi:10.1016/j.chemosphere.2020.128978 .
Radovanović, Jelena, Antonijević, Biljana, Kolarević, Stoimir, Milutinović-Smiljanić, Sanja, Mandić, Jelena, Vuković-Gačić, Branka, Bulat, Zorica, Ćurčić, Marijana, Kračun-Kolarević, Margareta, Sunjog, Karolina, Kostić-Vuković, Jovana, Jovanović Marić, Jovana, Antonijević-Miljaković, Evica, Đukić-Ćosić, Danijela, Buha-Đorđević, Aleksandra, Javorac, Dragana, Baralić, Katarina, Mandinić, Zoran, "Genotoxicity of fluoride subacute exposure in rats and selenium intervention" in Chemosphere, 266 (2021), https://doi.org/10.1016/j.chemosphere.2020.128978 . .