Show simple item record

dc.creatorBaralić, Katarina
dc.creatorJorgovanović, Dragica
dc.creatorŽivančević, Katarina
dc.creatorBuha-Đorđević, Aleksandra
dc.creatorAntonijević-Miljaković, Evica
dc.creatorMiljković, Milica
dc.creatorKotur-Stevuljević, Jelena
dc.creatorAntonijević, Biljana
dc.creatorĐukić-Ćosić, Danijela
dc.date.accessioned2020-12-29T10:27:48Z
dc.date.available2020-12-29T10:27:48Z
dc.date.issued2021
dc.identifier.issn0045-6535
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3762
dc.description.abstractThe aim of this study was to: (i) determine and compare the capacity of bis (2 –ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP), bisphenol A (BPA), and their mixture to produce testicular toxicity after the subacute exposure; (ii) explore the mechanisms behind the observed changes using in silico toxicogenomic approach. Male rats were randomly split into groups (n = 6): (1) Control (corn oil); (2) DEHP (50 mg/kg b.w./day); (3) DBP (50 mg/kg b.w./day); (4) BPA (25 mg/kg b.w./day); and (5) MIX (50 mg/kg b.w./day DEHP + 50 mg/kg b.w/day DBP + 25 mg/kg b.w./day BPA). Animals were sacrificed after 28 days of oral exposure, testes were extracted and prepared for histological assessments under the light microscope (haematoxylin and eosin staining) and redox status analysis. The Comparative Toxicogenomics Database (CTD; http://CTD.mdibl.org), Cytoscape software (https://cytoscape.org) and ToppGene Suite (https://toppgene.cchmc.org) were used for data-mining. Present pathohistological study has demonstrated more pronounced testicular toxicity of the MIX group (desquamated germinal epithelium cells, enlarged cells with hyperchromatic nuclei, multinucleated cell forms and intracytoplasmic vacuoles) in comparison with the single substances, while effects on redox status parameters were either more prominent, or present only in the MIX group. In silico investigation revealed 20 genes linked to male reproductive disorders, affected by all three investigated substances. Effects on metabolism, AhR pathway, apoptosis and oxidative stress could be singled out as the most probable mechanisms involved in the subacute DEHP, DBP and BPA mixture testicular toxicity, while the effect on oxidative stress parameters was confirmed by in vivo experiment.
dc.publisherElsevier Ltd
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.rightsrestrictedAccess
dc.sourceChemosphere
dc.subjectRats
dc.subjectEndocrine disruptors
dc.subjectTesticular toxicity
dc.subjectToxicogenomic data-mining
dc.titleCombining in vivo pathohistological and redox status analysis with in silico toxicogenomic study to explore the phthalates and bisphenol A mixture-induced testicular toxicity
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЂукић-Ћосић, Данијела; Баралић, Катарина; Јорговановић, Драгица; Миљковић, Милица; Котур-Стевуљевић, Јелена; Aнтонијевић, Биљана; Живанчевић, К.; Буха-Ђјорђевић, Aлександра; Aнтонијевић-Миљаковић, Евица;
dc.citation.volume267
dc.citation.rankM21~
dc.identifier.doi10.1016/j.chemosphere.2020.129296
dc.identifier.scopus2-s2.0-85097792213
dc.type.versionpublishedVersion


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record