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Toksičnost organskog i neogranskog nikla u ćelijskim kulturama pankreasa: poređenje sa kadmijumom

dc.creatorWallace, David R.
dc.creatorBuha-Đorđević, Aleksandra
dc.creatorBenton, Alexander
dc.date.accessioned2021-01-25T13:11:15Z
dc.date.available2021-01-25T13:11:15Z
dc.date.issued2020
dc.identifier.issn0004-1963
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3770
dc.description.abstractNickel compounds are Group 1 carcinogens and possibly cancer-causing in the pancreas. We examined the toxicity of nickel in both 2-D and 3-D pancreatic cell cultures, to determine the LD50 for organic and inorganic nickel in normal and cancerous cells. Assays with cadmium chloride were performed to be a comparison to potential nickel-induced toxicity. Cells were exposed to twelve concentrations of NiCl2 or Ni-(Ac)2 for 48h (2-D), or six concentrations for 48 hours (3-D). There was a significant (P=0.0016) difference between HPNE and AsPC-1 LD50values after cadmium exposure, at 69.9 μM and 29.2 μM, respectively. Neither form of nickel exhibited toxicity in 2-D or 3-D cultures, but after 48h, changes in spheroid morphology were observed. The inability of Ni to reduce viable cell numbers suggests a toxic mechanism that differs from cadmium, also a Group 1 carcinogen. The cell microenvironment was not a factor in nickel toxicity with no changes in viable cells in either 2-D or 3-D cultures. These studies only examined cytotoxicity, and not genotoxicity, a potential mechanism of nickel carcinogenicity. Alterations in DNA function or the expression of apoptotic proteins/processes would take longer to manifest. Current work focuses on cellular changes following extended nickel exposure.sr
dc.description.abstractUloga nikla, toksičnog metala, u nastanku karcinoma pankreasa još uvek nije u potpunosti ispitana. Cilj rada je da ispita toksičnost nikla (Ni) u 2-D i 3-D kulturi ćelija pankreasa, kako bi se utvrdila LD50 vrednost za organski i neorganski nikl u normalnim i tumorskim ćelijama. Ispitivanja su izvršena i sa kadmijumom (Cd), metalom čija je uloga u nastanku karcinoma pankreasa potvrđena u prethodnim istraživanjima, a u svrhu poređenja sa potencijalnom toksičnošću izazvanom Ni. Ćelije su bile tretirane sa 12 različitih koncentracija NiCl2 ili Ni-(Ac)2 tokom 48h (2-D), odnosno sa šest različitih koncentracija tokom 48 sati (3-D). Nijedan oblik Ni nije ispoljio toksičnost u 2-D ili 3-D kulturama, ali nakon 48h primećene su promene u sferoidnoj morfologiji. Nemogućnost Ni da smanji broj vijabilnih ćelija sugeriše mehanizam karcinogeneze različit od mehanizma koji ispoljava Cd. Ipak, da bi se uočile izmene u funkciji DNK ili izražavanju apoptotičkih proteina/procesa potrebno je duže vremena, pa su dalja istraživanja upravo fokusirana na ćelijske promene nakon produžene izloženosti nikla.sr
dc.language.isoensr
dc.publisherPharmaceutical Association of Serbiasr
dc.relationOklahoma IDeA Network of Biomedical Research Excellence (OK-INBRE; P20GM103447; DRW)sr
dc.relationNational Institutes of Health and the OSU-CHS 355 Office of the Vice President for Research Seed Grant (#1-54333; DRW)sr
dc.rightsopenAccesssr
dc.sourceArhiv za farmacijusr
dc.subjectnickelsr
dc.subjectcadmiumsr
dc.subjectcytotoxicitysr
dc.subjecttoxic metalssr
dc.subjectcancersr
dc.subjectniklsr
dc.subjectkadmijumsr
dc.subjectcitotoksičnostsr
dc.subjecttoksični metalisr
dc.subjectkarcinomsr
dc.titleToxicity of Organic and Inorganic Nickel in Pancreatic Cell Cultures: Comparison to Cadmiumsr
dc.titleToksičnost organskog i neogranskog nikla u ćelijskim kulturama pankreasa: poređenje sa kadmijumomsr
dc.typearticlesr
dc.rights.licenseBY-SAsr
dcterms.abstractWаллаце, Давид Р.; Буха-Ђорђевић, Aлександра; Бентон, Aлеxандер; Токсичност органског и неогранског никла у ћелијским културама панкреаса: поређење са кадмијумом; Токсичност органског и неогранског никла у ћелијским културама панкреаса: поређење са кадмијумом;
dc.citation.volume70
dc.citation.issue6
dc.citation.spage344
dc.citation.epage359
dc.citation.rankM52~
dc.identifier.doi10.5937/arhfarm70-29277
dc.identifier.scopus2-s2.0-85100161048
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs/bitstream/id/8552/Toxicity_of_Organic_pub_2020.pdf
dc.type.versionpublishedVersionsr


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