Toxicity of Organic and Inorganic Nickel in Pancreatic Cell Cultures: Comparison to Cadmium
Toksičnost organskog i neogranskog nikla u ćelijskim kulturama pankreasa: poređenje sa kadmijumom
dc.creator | Wallace, David R. | |
dc.creator | Buha-Đorđević, Aleksandra | |
dc.creator | Benton, Alexander | |
dc.date.accessioned | 2021-01-25T13:11:15Z | |
dc.date.available | 2021-01-25T13:11:15Z | |
dc.date.issued | 2020 | |
dc.identifier.issn | 0004-1963 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3770 | |
dc.description.abstract | Nickel compounds are Group 1 carcinogens and possibly cancer-causing in the pancreas. We examined the toxicity of nickel in both 2-D and 3-D pancreatic cell cultures, to determine the LD50 for organic and inorganic nickel in normal and cancerous cells. Assays with cadmium chloride were performed to be a comparison to potential nickel-induced toxicity. Cells were exposed to twelve concentrations of NiCl2 or Ni-(Ac)2 for 48h (2-D), or six concentrations for 48 hours (3-D). There was a significant (P=0.0016) difference between HPNE and AsPC-1 LD50values after cadmium exposure, at 69.9 μM and 29.2 μM, respectively. Neither form of nickel exhibited toxicity in 2-D or 3-D cultures, but after 48h, changes in spheroid morphology were observed. The inability of Ni to reduce viable cell numbers suggests a toxic mechanism that differs from cadmium, also a Group 1 carcinogen. The cell microenvironment was not a factor in nickel toxicity with no changes in viable cells in either 2-D or 3-D cultures. These studies only examined cytotoxicity, and not genotoxicity, a potential mechanism of nickel carcinogenicity. Alterations in DNA function or the expression of apoptotic proteins/processes would take longer to manifest. Current work focuses on cellular changes following extended nickel exposure. | sr |
dc.description.abstract | Uloga nikla, toksičnog metala, u nastanku karcinoma pankreasa još uvek nije u potpunosti ispitana. Cilj rada je da ispita toksičnost nikla (Ni) u 2-D i 3-D kulturi ćelija pankreasa, kako bi se utvrdila LD50 vrednost za organski i neorganski nikl u normalnim i tumorskim ćelijama. Ispitivanja su izvršena i sa kadmijumom (Cd), metalom čija je uloga u nastanku karcinoma pankreasa potvrđena u prethodnim istraživanjima, a u svrhu poređenja sa potencijalnom toksičnošću izazvanom Ni. Ćelije su bile tretirane sa 12 različitih koncentracija NiCl2 ili Ni-(Ac)2 tokom 48h (2-D), odnosno sa šest različitih koncentracija tokom 48 sati (3-D). Nijedan oblik Ni nije ispoljio toksičnost u 2-D ili 3-D kulturama, ali nakon 48h primećene su promene u sferoidnoj morfologiji. Nemogućnost Ni da smanji broj vijabilnih ćelija sugeriše mehanizam karcinogeneze različit od mehanizma koji ispoljava Cd. Ipak, da bi se uočile izmene u funkciji DNK ili izražavanju apoptotičkih proteina/procesa potrebno je duže vremena, pa su dalja istraživanja upravo fokusirana na ćelijske promene nakon produžene izloženosti nikla. | sr |
dc.language.iso | en | sr |
dc.publisher | Pharmaceutical Association of Serbia | sr |
dc.relation | Oklahoma IDeA Network of Biomedical Research Excellence (OK-INBRE; P20GM103447; DRW) | sr |
dc.relation | National Institutes of Health and the OSU-CHS 355 Office of the Vice President for Research Seed Grant (#1-54333; DRW) | sr |
dc.rights | openAccess | sr |
dc.rights.uri | https://creativecommons.org/licenses/by-sa/4.0/ | |
dc.source | Arhiv za farmaciju | sr |
dc.subject | nickel | sr |
dc.subject | cadmium | sr |
dc.subject | cytotoxicity | sr |
dc.subject | toxic metals | sr |
dc.subject | cancer | sr |
dc.subject | nikl | sr |
dc.subject | kadmijum | sr |
dc.subject | citotoksičnost | sr |
dc.subject | toksični metali | sr |
dc.subject | karcinom | sr |
dc.title | Toxicity of Organic and Inorganic Nickel in Pancreatic Cell Cultures: Comparison to Cadmium | sr |
dc.title | Toksičnost organskog i neogranskog nikla u ćelijskim kulturama pankreasa: poređenje sa kadmijumom | sr |
dc.type | article | sr |
dc.rights.license | BY-SA | sr |
dcterms.abstract | Wаллаце, Давид Р.; Буха-Ђорђевић, Aлександра; Бентон, Aлеxандер; Токсичност органског и неогранског никла у ћелијским културама панкреаса: поређење са кадмијумом; Токсичност органског и неогранског никла у ћелијским културама панкреаса: поређење са кадмијумом; | |
dc.citation.volume | 70 | |
dc.citation.issue | 6 | |
dc.citation.spage | 344 | |
dc.citation.epage | 359 | |
dc.citation.rank | M51 | |
dc.identifier.doi | 10.5937/arhfarm70-29277 | |
dc.identifier.scopus | 2-s2.0-85100161048 | |
dc.identifier.fulltext | https://farfar.pharmacy.bg.ac.rs/bitstream/id/8552/Toxicity_of_Organic_pub_2020.pdf | |
dc.type.version | publishedVersion | sr |