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dc.creatorŽivković, Lada
dc.creatorBajić, Vladan
dc.creatorČabarkapa-Pirković, Andrea
dc.creatorDekanski, Dragana
dc.creatorForbes-Hernández, Tamara
dc.creatorZlatković-Švenda, Mirjana
dc.creatorPerry, George
dc.creatorSpremo-Potparević, Biljana
dc.date.accessioned2021-02-23T09:02:08Z
dc.date.available2021-02-23T09:02:08Z
dc.date.issued2021
dc.identifier.issn0145-8884
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3784
dc.description.abstractIncreased levels of oxidative stress and oxidative DNA damage are common features in the pathology of Alzheimer’s disease (AD) found in neurons and peripheral cells like peripheral blood lymphocytes (PBL). Natural products such as strawberry cultivar Alba are an important source of bioactive nutrients that could help in lowering both the oxidative stress and DNA damage levels. The objective was to estimate the effects of Alba extract on DNA damage in peripheral blood lymphocytes of sporadic AD (aged 60–84 years) patients, and healthy elderly (aged 69–83 years) and young (aged 21–30 years) individuals in in vitro conditions. Comet assay was used as a sensitive technique for the evaluation of PBL DNA damage levels. Reduction of basal DNA damage level in PBL was shown in the young group after the incubation with Alba extract ranging from 25 to 200 μg/ml, with 100 μg/ml being the most effective concentration. Selected Alba extract of 100 μg/ml was further used for PBL treatment of AD and healthy elderly age matched group, displaying potential to significantly attenuate DNA damage levels in both groups (p <.05). Alba extract displayed biological activity against oxidative DNA damage, suggesting that its functional ingredients may have beneficial health effects. Practical applications: The data obtained in this preliminary study displayed that strawberry Alba extract is efficient against DNA damage induced by endogenous and exogenous oxidative stress in peripheral blood lymphocytes of Alzheimer`s disease in vitro. An active area of future research of Alba cultivar should be to determine the trials in in vivo systems. Our findings also suggest that Alba cultivar’s functional ingredients potentially may have beneficial health effects in AD.
dc.publisherWiley
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.relationCOMET COST action (15132)
dc.rightsrestrictedAccess
dc.sourceJournal of Food Biochemistry
dc.subjectDNA damage
dc.subjectageing
dc.subjectcomet assay
dc.subjectneurodegenerative disease
dc.titleStrawberry (Fragaria ananassa duch.) Alba extract attenuates DNA damage in lymphocytes of patients with Alzheimer’s disease
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЖивковић, Лада; Бајић, Владан; Декански, Драгана; Перрy, Георге; Спремо-Потпаревић, Биљана; Форбес-Хернáндез, Тамара; Чабаркапа-Пирковић, Aндреа; Златковић-Швенда, Мирјана;
dc.citation.volume45
dc.citation.issue4
dc.identifier.wos000615098900001
dc.identifier.doi10.1111/jfbc.13637
dc.identifier.scopus2-s2.0-85100577124
dc.type.versionpublishedVersion


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