The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
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2021
Authors
Milić, MirtaCeppi, Marcello
Bruzzone, Marco
Azqueta, Amaya
Brunborg, Gunnar
Godschalk, Roger
Koppen, Gudrun
Langie, Sabine
Møller, Peter
Teixeira, João Paulo
Alija, Avdulla
Anderson, Diana
Andrade, Vanessa
Andreoli, Cristina
Asllani, Fisnik
Bangkoglu, Ezgi Eyluel
Barančoková, Magdalena
Basaran, Nursen
Boutet-Robinet, Elisa
Buschini, Annamaria
Cavallo, Delia
Costa Pereira, Cristiana
Costa, Carla
Costa, Solange
Da Silva, Juliana
Del Boˊ, Cristian
Dimitrijević Srećković, Vesna
Đelić, Ninoslav
Dobrzyńska, Malgorzata
Duračková, Zdenka
Dvořáková, Monika
Gajski, Goran
Galati, Serena
García Lima, Omar
Giovannelli, Lisa
Goroshinskaya, Irina A.
Grindel, Annemarie
Gutzkow, Kristine B.
Hernández, Alba
Hernández, Carlos
Holven, Kirsten B.
Ibero-Baraibar, Idoia
Ottestad, Inger
Kadioglu, Ela
Kažimirová, Alena
Kuznetsova, Elena
Ladeira, Carina
Laffon, Blanca
Lamonaca, Palma
Lebailly, Pierre
Louro, Henriqueta
Mandina Cardoso, Tania
Marcon, Francesca
Marcos, Ricard
Moretti, Massimo
Moretti, Silvia
Najafzadeh, Mojgan
Nemeth, Zsuzsanna
Neri, Monica
Novotna, Bozena
Orlow, Irene
Paduchova, Zuzana
Pastor, Susana
Perdry, Hervé
Spremo-Potparević, Biljana

Ramadhani, Dwi
Riso, Patrizia
Rohr, Paula
Rojas, Emilio
Rossner, Pavel
Safar, Anna
Sardas, Semra
Silva, Maria João
Sirota, Nikolay
Smolkova, Bozena
Staruchova, Marta
Stetina, Rudolf
Stopper, Helga
Surikova, Ekaterina I.
Ulven, Stine M.
Ursini, Cinzia Lucia
Valdiglesias, Vanessa

Valverde, Mahara
Vodicka, Pavel
Volkovova, Katarina
Wagner, Karl-Heinz
Živković, Lada

Dušinská, Maria
Collins, Andrew R.
Bonassi, Stefano
Article (Published version)
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Show full item recordAbstract
The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, sp...ecimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
Keywords:
DNA damage / Biomarkers / Comet assay / Human biomonitoring / Pooled analysisSource:
Mutation Research - Reviews in Mutation Research, 2021, 787Publisher:
- Elsevier B.V.
Funding / projects:
- Cell Cycle Aberrations and the Impact of Oxidative Stress in Neurodegenerative Processes and Malignant Transformation of the Cell (RS-173034)
- Interactive role of dyslipidemia, oxidative stress and inflammation in atherosclerosis and other diseases: genetic and biochemical markers (RS-175035)
- Molecular genetic and ecophysiological researches on the protection of autochthonous animal genetic resources, sustaining domestic animals’ welfare, health and reproduction, and safe food production (RS-46002)
DOI: 10.1016/j.mrrev.2021.108371
ISSN: 1383-5742
WoS: 000658539600011
Scopus: 2-s2.0-85101040695
Collections
Institution/Community
PharmacyTY - JOUR AU - Milić, Mirta AU - Ceppi, Marcello AU - Bruzzone, Marco AU - Azqueta, Amaya AU - Brunborg, Gunnar AU - Godschalk, Roger AU - Koppen, Gudrun AU - Langie, Sabine AU - Møller, Peter AU - Teixeira, João Paulo AU - Alija, Avdulla AU - Anderson, Diana AU - Andrade, Vanessa AU - Andreoli, Cristina AU - Asllani, Fisnik AU - Bangkoglu, Ezgi Eyluel AU - Barančoková, Magdalena AU - Basaran, Nursen AU - Boutet-Robinet, Elisa AU - Buschini, Annamaria AU - Cavallo, Delia AU - Costa Pereira, Cristiana AU - Costa, Carla AU - Costa, Solange AU - Da Silva, Juliana AU - Del Boˊ, Cristian AU - Dimitrijević Srećković, Vesna AU - Đelić, Ninoslav AU - Dobrzyńska, Malgorzata AU - Duračková, Zdenka AU - Dvořáková, Monika AU - Gajski, Goran AU - Galati, Serena AU - García Lima, Omar AU - Giovannelli, Lisa AU - Goroshinskaya, Irina A. AU - Grindel, Annemarie AU - Gutzkow, Kristine B. AU - Hernández, Alba AU - Hernández, Carlos AU - Holven, Kirsten B. AU - Ibero-Baraibar, Idoia AU - Ottestad, Inger AU - Kadioglu, Ela AU - Kažimirová, Alena AU - Kuznetsova, Elena AU - Ladeira, Carina AU - Laffon, Blanca AU - Lamonaca, Palma AU - Lebailly, Pierre AU - Louro, Henriqueta AU - Mandina Cardoso, Tania AU - Marcon, Francesca AU - Marcos, Ricard AU - Moretti, Massimo AU - Moretti, Silvia AU - Najafzadeh, Mojgan AU - Nemeth, Zsuzsanna AU - Neri, Monica AU - Novotna, Bozena AU - Orlow, Irene AU - Paduchova, Zuzana AU - Pastor, Susana AU - Perdry, Hervé AU - Spremo-Potparević, Biljana AU - Ramadhani, Dwi AU - Riso, Patrizia AU - Rohr, Paula AU - Rojas, Emilio AU - Rossner, Pavel AU - Safar, Anna AU - Sardas, Semra AU - Silva, Maria João AU - Sirota, Nikolay AU - Smolkova, Bozena AU - Staruchova, Marta AU - Stetina, Rudolf AU - Stopper, Helga AU - Surikova, Ekaterina I. AU - Ulven, Stine M. AU - Ursini, Cinzia Lucia AU - Valdiglesias, Vanessa AU - Valverde, Mahara AU - Vodicka, Pavel AU - Volkovova, Katarina AU - Wagner, Karl-Heinz AU - Živković, Lada AU - Dušinská, Maria AU - Collins, Andrew R. AU - Bonassi, Stefano PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3791 AB - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations. PB - Elsevier B.V. T2 - Mutation Research - Reviews in Mutation Research T1 - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders VL - 787 DO - 10.1016/j.mrrev.2021.108371 ER -
@article{ author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano", year = "2021", abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.", publisher = "Elsevier B.V.", journal = "Mutation Research - Reviews in Mutation Research", title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders", volume = "787", doi = "10.1016/j.mrrev.2021.108371" }
Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research Elsevier B.V.., 787. https://doi.org/10.1016/j.mrrev.2021.108371
Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research. 2021;787. doi:10.1016/j.mrrev.2021.108371 .
Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research - Reviews in Mutation Research, 787 (2021), https://doi.org/10.1016/j.mrrev.2021.108371 . .