Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals
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2020
Authors
Janać, Jelena
Zeljković, Aleksandra

Jelić-Ivanović, Zorana

Dimitrijević-Srećković, Vesna
Vekić, Jelena

Miljković, Milica

Stefanović, Aleksandra

Kotur-Stevuljević, Jelena

Ivanišević, Jasmina

Spasojević-Kalimanovska, Vesna

Article (Published version)

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Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verifi...ed by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.
Keywords:
HDL-cholesterol / Lipoprotein subfractions / Metabolically healthy phenotype / Overweight and obesity / Oxidized HDL / Serum amyloid ASource:
Lab Medicine, 2020, 51, 1, 24-33Publisher:
- Oxford University Press
Funding / projects:
DOI: 10.1093/labmed/lmz017
ISSN: 0007-5027
WoS: 000562343700006
Scopus: 2-s2.0-85077402988
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PharmacyTY - JOUR AU - Janać, Jelena AU - Zeljković, Aleksandra AU - Jelić-Ivanović, Zorana AU - Dimitrijević-Srećković, Vesna AU - Vekić, Jelena AU - Miljković, Milica AU - Stefanović, Aleksandra AU - Kotur-Stevuljević, Jelena AU - Ivanišević, Jasmina AU - Spasojević-Kalimanovska, Vesna PY - 2020 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3803 AB - Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals. PB - Oxford University Press T2 - Lab Medicine T1 - Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals VL - 51 IS - 1 SP - 24 EP - 33 DO - 10.1093/labmed/lmz017 ER -
@article{ author = "Janać, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Dimitrijević-Srećković, Vesna and Vekić, Jelena and Miljković, Milica and Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Spasojević-Kalimanovska, Vesna", year = "2020", abstract = "Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.", publisher = "Oxford University Press", journal = "Lab Medicine", title = "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals", volume = "51", number = "1", pages = "24-33", doi = "10.1093/labmed/lmz017" }
Janać, J., Zeljković, A., Jelić-Ivanović, Z., Dimitrijević-Srećković, V., Vekić, J., Miljković, M., Stefanović, A., Kotur-Stevuljević, J., Ivanišević, J.,& Spasojević-Kalimanovska, V.. (2020). Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine Oxford University Press., 51(1), 24-33. https://doi.org/10.1093/labmed/lmz017
Janać J, Zeljković A, Jelić-Ivanović Z, Dimitrijević-Srećković V, Vekić J, Miljković M, Stefanović A, Kotur-Stevuljević J, Ivanišević J, Spasojević-Kalimanovska V. Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine. 2020;51(1):24-33. doi:10.1093/labmed/lmz017 .
Janać, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Dimitrijević-Srećković, Vesna, Vekić, Jelena, Miljković, Milica, Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Spasojević-Kalimanovska, Vesna, "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals" in Lab Medicine, 51, no. 1 (2020):24-33, https://doi.org/10.1093/labmed/lmz017 . .