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dc.creatorĆupić Miladinović, Dejana
dc.creatorPrevendar Crnić, Andreja
dc.creatorPeković, Sanja
dc.creatorDacić, Sanja
dc.creatorIvanović, Saša
dc.creatorSantibanez, Juan Francisco
dc.creatorĆupić, Vitomir
dc.creatorBorozan, Nevena
dc.creatorAntonijević-Miljaković, Evica
dc.creatorBorozan, Sunčica
dc.date.accessioned2021-04-01T08:14:59Z
dc.date.available2021-04-01T08:14:59Z
dc.date.issued2021
dc.identifier.issn0009-2797
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3810
dc.description.abstractChlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
dc.publisherElsevier Ireland Ltd
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200143/RS//
dc.rightsrestrictedAccess
dc.sourceChemico-Biological Interactions
dc.subjectOxidative stress
dc.subjectBrain
dc.subjectChlorpyrifos
dc.subjectInflammation
dc.subjectJapanese quail
dc.subjectVitamin B1
dc.titleRecovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration
dc.typearticle
dc.rights.licenseARR
dcterms.abstractAнтонијевић-Миљаковић, Евица; Ћупић, Витомир; Борозан, Сунчица; Ћупић Миладиновић, Дејана; Превендар Црнић, Aндреја; Пековић, Сања; Дацић, Сања; Ивановић, Саша; Сантибанез, Јуан Францисцо; Борозан, Невена;
dc.citation.volume333
dc.citation.rankM21
dc.identifier.wos000600832900017
dc.identifier.doi10.1016/j.cbi.2020.109312
dc.identifier.scopus2-s2.0-85096159138
dc.type.versionpublishedVersion


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