Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes
Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka
Authors
Spremo-Potparević, BiljanaČabarkapa-Pirković, Andrea
Dekanski, Dragana
Topalović, Dijana
Bajić, Vladan
Živković, Lada
Article (Published version)
Metadata
Show full item recordAbstract
Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results ind...icate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.
Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasa...n u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.
Keywords:
DNA damage / comet assay / strawberry (cv. Romina) / anthocyanins / leukocytes / oštećenje DNK / komet test / jagoda (cv. Romina) / antocijanini / leukocitiSource:
Arhiv za farmaciju, 2021, 71, 3, 197-206Publisher:
- Beograd : Savez farmaceutskih udruženja Srbije
Funding / projects:
- Ministry of Science, Technological Development and Innovation of the Republic of Serbia, institutional funding - 200161 (University of Belgrade, Faculty of Pharmacy) (RS-MESTD-inst-2020-200161)
Collections
Institution/Community
PharmacyTY - JOUR AU - Spremo-Potparević, Biljana AU - Čabarkapa-Pirković, Andrea AU - Dekanski, Dragana AU - Topalović, Dijana AU - Bajić, Vladan AU - Živković, Lada PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3920 AB - Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent. AB - Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo. PB - Beograd : Savez farmaceutskih udruženja Srbije T2 - Arhiv za farmaciju T1 - Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes T1 - Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka VL - 71 IS - 3 SP - 197 EP - 206 DO - 10.5937/arhfarm71‐31060 ER -
@article{ author = "Spremo-Potparević, Biljana and Čabarkapa-Pirković, Andrea and Dekanski, Dragana and Topalović, Dijana and Bajić, Vladan and Živković, Lada", year = "2021", abstract = "Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxidein vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 μg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposureof the leukocytes to the ACY, and subsequent incubation with H2O2, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 μg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent., Plodovi jagode Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), bogati antocijaninima, pokazali su povoljne efekte na zdravlje zahvaljujući svojim antioksidativnim svojstvima. U ovoj studiji ispitivan je antigenotoksični potencijal frakcije metanolnog ekstrakta jagode Romina koji je obogaćen antocijaninom (ACY) na oštećenja DNK na leukocitima humane periferne krvi, izazvanih vodonik-peroksidom in vitro. U svim eksperimentima je korišćeno i testirano pet koncentracija ekstrakta ACY (2.5, 5, 10, 15, 20 µg /ml). Rezultati ispitivanja alkalnim komet testom nisu pokazali genotoksični efekat ACY. Nakon izloženosti leukocita ACY i potom njihovog tretmana i inkubacije sa H2O2, smanjen je broj ćelija sa oštećenom DNK, u svim ispitivanim koncentracijama u poređenju sa kontrolama. U post-tretmanu, došlo je do smanjenja oštećenja DNK u zavisnosti od koncentracije ekstrakta, dok je statistički značajno smanjenje postignuto sa koncentracijama od 15 i 20 µg/ml. Rezultati ukazuju da je ACY efikasan u smanjenju oksidativnih oštećenja DNK i da pokazuje snažniji efekat kao postaplikativno nego kao zaštitno sredstvo.", publisher = "Beograd : Savez farmaceutskih udruženja Srbije", journal = "Arhiv za farmaciju", title = "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes, Antigenotoksična svojstva ekstrakta jagode obogaćenog antocijaninom (cv. Romina) na oštećenja DNK izazvana H2O2 u leukocitima periferne krvi čoveka", volume = "71", number = "3", pages = "197-206", doi = "10.5937/arhfarm71‐31060" }
Spremo-Potparević, B., Čabarkapa-Pirković, A., Dekanski, D., Topalović, D., Bajić, V.,& Živković, L.. (2021). Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju Beograd : Savez farmaceutskih udruženja Srbije., 71(3), 197-206. https://doi.org/10.5937/arhfarm71‐31060
Spremo-Potparević B, Čabarkapa-Pirković A, Dekanski D, Topalović D, Bajić V, Živković L. Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes. in Arhiv za farmaciju. 2021;71(3):197-206. doi:10.5937/arhfarm71‐31060 .
Spremo-Potparević, Biljana, Čabarkapa-Pirković, Andrea, Dekanski, Dragana, Topalović, Dijana, Bajić, Vladan, Živković, Lada, "Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes" in Arhiv za farmaciju, 71, no. 3 (2021):197-206, https://doi.org/10.5937/arhfarm71‐31060 . .