Приказ основних података о документу
Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches
dc.creator | Račić, Andjelka | |
dc.creator | Čalija, Bojan | |
dc.creator | Milić, Jela | |
dc.creator | Jurišić Dukovski, Bisera | |
dc.creator | Lovrić, Jasmina | |
dc.creator | Dobričić, Vladimir | |
dc.creator | Micov, Ana | |
dc.creator | Vuković, Milja | |
dc.creator | Stepanović-Petrović, Radica | |
dc.creator | Krajišnik, Danina | |
dc.date.accessioned | 2021-08-24T10:09:14Z | |
dc.date.available | 2021-08-24T10:09:14Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 0928-0987 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3938 | |
dc.description.abstract | The aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine. | |
dc.publisher | Elsevier B.V. | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS// | |
dc.rights | restrictedAccess | |
dc.source | European Journal of Pharmaceutical Sciences | |
dc.subject | HCE-T cell-based models | |
dc.subject | MTT cytotoxicity assay | |
dc.subject | Ocular itch test | |
dc.subject | Olopatadine hydrochloride | |
dc.subject | PAMPA | |
dc.subject | Viscous eye drops | |
dc.title | Formulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches | |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Рачић, Aндјелка; Ловрић, Јасмина; Чалија, Бојан; Милић, Јела; Јуришић Дуковски, Бисера; Добричић, Владимир; Мицов, Aна; Вуковић, Миља; Степановић-Петровић, Радица; Крајишник, Данина; | |
dc.citation.volume | 166 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 000704195200003 | |
dc.identifier.doi | 10.1016/j.ejps.2021.105906 | |
dc.identifier.scopus | 2-s2.0-85112542192 | |
dc.type.version | publishedVersion |