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dc.creatorRačić, Andjelka
dc.creatorČalija, Bojan
dc.creatorMilić, Jela
dc.creatorJurišić Dukovski, Bisera
dc.creatorLovrić, Jasmina
dc.creatorDobričić, Vladimir
dc.creatorMicov, Ana
dc.creatorVuković, Milja
dc.creatorStepanović-Petrović, Radica
dc.creatorKrajišnik, Danina
dc.date.accessioned2021-08-24T10:09:14Z
dc.date.available2021-08-24T10:09:14Z
dc.date.issued2021
dc.identifier.issn0928-0987
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/3938
dc.description.abstractThe aim of this work was the formulation and the comprehensive evaluation of the viscous eye drops using vehicles containing medium chain chitosan (0.5% w/v), hydroxypropyl guar gum (0.25% w/v) and their com-bination as carriers for olopatadine (0.1% w/v). Physicochemical properties (appearance, clarity, pH, osmolality, viscosity and drug content) of the tested formulations were within acceptable ranges for the ophthalmic prep-arations, while DSC and FT-IR techniques demonstrated the compatibility between olopatadine and polymers. The drug permeability was successfully estimated in vitro using both HCE-T cell-based models (Model I and Model II) and the parallel artificial membrane permeability assay (PAMPA), considering the impact of chitosan as a permeation enhancer. The MTT cytotoxicity assay demonstrates that the tested formulations (diluted 10-fold in HBSS pH 5.5) were non-toxic and well tolerated. An ocular itch test on mice was carried out with the formulation containing the combination of polymers comparable with a commercially available olopatadine eye drops without viscosity enhancers. The tested eye drops produced a slightly higher anti-pruritic/analgesic-like effect than the commercial preparation. It could be assumed that the use of this viscous ophthalmic vehicle due to its advanced mucoadhesive properties and good safety profile is a feasible strategy to improve the efficacy of olopatadine.
dc.publisherElsevier B.V.
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.rightsrestrictedAccess
dc.sourceEuropean Journal of Pharmaceutical Sciences
dc.subjectHCE-T cell-based models
dc.subjectMTT cytotoxicity assay
dc.subjectOcular itch test
dc.subjectOlopatadine hydrochloride
dc.subjectPAMPA
dc.subjectViscous eye drops
dc.titleFormulation of olopatadine hydrochloride viscous eye drops – physicochemical, biopharmaceutical and efficacy assessment using in vitro and in vivo approaches
dc.typearticle
dc.rights.licenseARR
dcterms.abstractРачић, Aндјелка; Ловрић, Јасмина; Чалија, Бојан; Милић, Јела; Јуришић Дуковски, Бисера; Добричић, Владимир; Мицов, Aна; Вуковић, Миља; Степановић-Петровић, Радица; Крајишник, Данина;
dc.citation.volume166
dc.citation.rankM21
dc.identifier.wos000704195200003
dc.identifier.doi10.1016/j.ejps.2021.105906
dc.identifier.scopus2-s2.0-85112542192
dc.type.versionpublishedVersion


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Приказ основних података о документу