DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death
Аутори
Bonassi, StefanoCeppi, Marcello
Møller, Peter
Azqueta, Amaya
Milić, Mirta
Monica, Neri
Brunborg, Gunnar
Godschalk, Roger
Koppen, Gudrun
Langie, Sabine A. S.
Teixeira, João Paulo
Bruzzone, , Marco
Da Silva, Juliana
Benedetti, Danieli
Cavallo, Delia
Ursini, Cinzia Lucia
Giovannelli, Lisa
Moretti, Silvia
Riso, Patrizia
Del Bo’, Cristian
Russo, Patrizia
Dobrzyńska, , Malgorzata
Goroshinskaya, Irina A.
Surikova, Ekaterina I.
Staruchova, Marta
Barančokova, Magdalena
Volkovova, Katarina
Kažimirova, Alena
Smolkova, Bozena
Laffon, Blanca
Valdiglesias, Vanessa
Pastor, Susana
Marcos, Ricard
Hernández, Alba
Gajski, Goran
Spremo-Potparević, Biljana
Živković, Lada
Boutet-Robinet, Elisa
Perdry, Hervé
Lebailly, Pierre
Perez, Carlos L.
Basaran, Nursen
Nemeth, , Zsuzsanna
Safar, Anna
Dusinska, Maria
Collins, Andrew
Anderson, Diana
Andrade, Vanessa
Pereira, Cristiana Costa
Costa, Solange
Gutzkow, Kristine B.
Ladeira, Carina
Moretti, Massimo
Costa, Carla
Orlow, Irene
Rojas, Emilio
Pourrut, Bertrand
Kruszewski, Marcin
Knasmueller, Siegfried
Shaposhnikov, Sergey
Žegura, Bojana
Stopper, Helga
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.
Извор:
Scientific Reports, 2021, 11, 1Издавач:
- Nature Research
Финансирање / пројекти:
- COST Action CA15132 (hCOMET), supported by COST (European Cooperation in Science and Technology).
- Italian Ministry of Health for Institutional Research (Ricerca Corrente).
Напомена:
- This article has been corrected. Link to the correction: https://farfar.pharmacy.bg.ac.rs/handle/123456789/3975
Повезане информације:
- Повезани садржај
https://farfar.pharmacy.bg.ac.rs/handle/123456789/3975
DOI: 10.1038/s41598-021-95976-7
ISSN: 2045-2322
WoS: 000686708000026
Scopus: 2-s2.0-85113162489
Институција/група
PharmacyTY - JOUR AU - Bonassi, Stefano AU - Ceppi, Marcello AU - Møller, Peter AU - Azqueta, Amaya AU - Milić, Mirta AU - Monica, Neri AU - Brunborg, Gunnar AU - Godschalk, Roger AU - Koppen, Gudrun AU - Langie, Sabine A. S. AU - Teixeira, João Paulo AU - Bruzzone, , Marco AU - Da Silva, Juliana AU - Benedetti, Danieli AU - Cavallo, Delia AU - Ursini, Cinzia Lucia AU - Giovannelli, Lisa AU - Moretti, Silvia AU - Riso, Patrizia AU - Del Bo’, Cristian AU - Russo, Patrizia AU - Dobrzyńska, , Malgorzata AU - Goroshinskaya, Irina A. AU - Surikova, Ekaterina I. AU - Staruchova, Marta AU - Barančokova, Magdalena AU - Volkovova, Katarina AU - Kažimirova, Alena AU - Smolkova, Bozena AU - Laffon, Blanca AU - Valdiglesias, Vanessa AU - Pastor, Susana AU - Marcos, Ricard AU - Hernández, Alba AU - Gajski, Goran AU - Spremo-Potparević, Biljana AU - Živković, Lada AU - Boutet-Robinet, Elisa AU - Perdry, Hervé AU - Lebailly, Pierre AU - Perez, Carlos L. AU - Basaran, Nursen AU - Nemeth, , Zsuzsanna AU - Safar, Anna AU - Dusinska, Maria AU - Collins, Andrew AU - Anderson, Diana AU - Andrade, Vanessa AU - Pereira, Cristiana Costa AU - Costa, Solange AU - Gutzkow, Kristine B. AU - Ladeira, Carina AU - Moretti, Massimo AU - Costa, Carla AU - Orlow, Irene AU - Rojas, Emilio AU - Pourrut, Bertrand AU - Kruszewski, Marcin AU - Knasmueller, Siegfried AU - Shaposhnikov, Sergey AU - Žegura, Bojana AU - Stopper, Helga PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3944 AB - The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases. PB - Nature Research T2 - Scientific Reports T1 - DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death VL - 11 IS - 1 DO - 10.1038/s41598-021-95976-7 ER -
@article{ author = "Bonassi, Stefano and Ceppi, Marcello and Møller, Peter and Azqueta, Amaya and Milić, Mirta and Monica, Neri and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine A. S. and Teixeira, João Paulo and Bruzzone, , Marco and Da Silva, Juliana and Benedetti, Danieli and Cavallo, Delia and Ursini, Cinzia Lucia and Giovannelli, Lisa and Moretti, Silvia and Riso, Patrizia and Del Bo’, Cristian and Russo, Patrizia and Dobrzyńska, , Malgorzata and Goroshinskaya, Irina A. and Surikova, Ekaterina I. and Staruchova, Marta and Barančokova, Magdalena and Volkovova, Katarina and Kažimirova, Alena and Smolkova, Bozena and Laffon, Blanca and Valdiglesias, Vanessa and Pastor, Susana and Marcos, Ricard and Hernández, Alba and Gajski, Goran and Spremo-Potparević, Biljana and Živković, Lada and Boutet-Robinet, Elisa and Perdry, Hervé and Lebailly, Pierre and Perez, Carlos L. and Basaran, Nursen and Nemeth, , Zsuzsanna and Safar, Anna and Dusinska, Maria and Collins, Andrew and Anderson, Diana and Andrade, Vanessa and Pereira, Cristiana Costa and Costa, Solange and Gutzkow, Kristine B. and Ladeira, Carina and Moretti, Massimo and Costa, Carla and Orlow, Irene and Rojas, Emilio and Pourrut, Bertrand and Kruszewski, Marcin and Knasmueller, Siegfried and Shaposhnikov, Sergey and Žegura, Bojana and Stopper, Helga", year = "2021", abstract = "The comet assay or single cell gel electrophoresis, is the most common method used to measure strand breaks and a variety of other DNA lesions in human populations. To estimate the risk of overall mortality, mortality by cause, and cancer incidence associated to DNA damage, a cohort of 2,403 healthy individuals (25,978 person-years) screened in 16 laboratories using the comet assay between 1996 and 2016 was followed-up. Kaplan–Meier analysis indicated a worse overall survival in the medium and high tertile of DNA damage (p < 0.001). The effect of DNA damage on survival was modelled according to Cox proportional hazard regression model. The adjusted hazard ratio (HR) was 1.42 (1.06–1.90) for overall mortality, and 1.94 (1.04–3.59) for diseases of the circulatory system in subjects with the highest tertile of DNA damage. The findings of this study provide epidemiological evidence encouraging the implementation of the comet assay in preventive strategies for non-communicable diseases.", publisher = "Nature Research", journal = "Scientific Reports", title = "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death", volume = "11", number = "1", doi = "10.1038/s41598-021-95976-7" }
Bonassi, S., Ceppi, M., Møller, P., Azqueta, A., Milić, M., Monica, N., Brunborg, G., Godschalk, R., Koppen, G., Langie, S. A. S., Teixeira, J. P., Bruzzone, ,. M., Da Silva, J., Benedetti, D., Cavallo, D., Ursini, C. L., Giovannelli, L., Moretti, S., Riso, P., Del Bo’, C., Russo, P., Dobrzyńska, ,. M., Goroshinskaya, I. A., Surikova, E. I., Staruchova, M., Barančokova, M., Volkovova, K., Kažimirova, A., Smolkova, B., Laffon, B., Valdiglesias, V., Pastor, S., Marcos, R., Hernández, A., Gajski, G., Spremo-Potparević, B., Živković, L., Boutet-Robinet, E., Perdry, H., Lebailly, P., Perez, C. L., Basaran, N., Nemeth, ,. Z., Safar, A., Dusinska, M., Collins, A., Anderson, D., Andrade, V., Pereira, C. C., Costa, S., Gutzkow, K. B., Ladeira, C., Moretti, M., Costa, C., Orlow, I., Rojas, E., Pourrut, B., Kruszewski, M., Knasmueller, S., Shaposhnikov, S., Žegura, B.,& Stopper, H.. (2021). DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports Nature Research., 11(1). https://doi.org/10.1038/s41598-021-95976-7
Bonassi S, Ceppi M, Møller P, Azqueta A, Milić M, Monica N, Brunborg G, Godschalk R, Koppen G, Langie SAS, Teixeira JP, Bruzzone ,M, Da Silva J, Benedetti D, Cavallo D, Ursini CL, Giovannelli L, Moretti S, Riso P, Del Bo’ C, Russo P, Dobrzyńska ,M, Goroshinskaya IA, Surikova EI, Staruchova M, Barančokova M, Volkovova K, Kažimirova A, Smolkova B, Laffon B, Valdiglesias V, Pastor S, Marcos R, Hernández A, Gajski G, Spremo-Potparević B, Živković L, Boutet-Robinet E, Perdry H, Lebailly P, Perez CL, Basaran N, Nemeth ,Z, Safar A, Dusinska M, Collins A, Anderson D, Andrade V, Pereira CC, Costa S, Gutzkow KB, Ladeira C, Moretti M, Costa C, Orlow I, Rojas E, Pourrut B, Kruszewski M, Knasmueller S, Shaposhnikov S, Žegura B, Stopper H. DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death. in Scientific Reports. 2021;11(1). doi:10.1038/s41598-021-95976-7 .
Bonassi, Stefano, Ceppi, Marcello, Møller, Peter, Azqueta, Amaya, Milić, Mirta, Monica, Neri, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine A. S., Teixeira, João Paulo, Bruzzone, , Marco, Da Silva, Juliana, Benedetti, Danieli, Cavallo, Delia, Ursini, Cinzia Lucia, Giovannelli, Lisa, Moretti, Silvia, Riso, Patrizia, Del Bo’, Cristian, Russo, Patrizia, Dobrzyńska, , Malgorzata, Goroshinskaya, Irina A., Surikova, Ekaterina I., Staruchova, Marta, Barančokova, Magdalena, Volkovova, Katarina, Kažimirova, Alena, Smolkova, Bozena, Laffon, Blanca, Valdiglesias, Vanessa, Pastor, Susana, Marcos, Ricard, Hernández, Alba, Gajski, Goran, Spremo-Potparević, Biljana, Živković, Lada, Boutet-Robinet, Elisa, Perdry, Hervé, Lebailly, Pierre, Perez, Carlos L., Basaran, Nursen, Nemeth, , Zsuzsanna, Safar, Anna, Dusinska, Maria, Collins, Andrew, Anderson, Diana, Andrade, Vanessa, Pereira, Cristiana Costa, Costa, Solange, Gutzkow, Kristine B., Ladeira, Carina, Moretti, Massimo, Costa, Carla, Orlow, Irene, Rojas, Emilio, Pourrut, Bertrand, Kruszewski, Marcin, Knasmueller, Siegfried, Shaposhnikov, Sergey, Žegura, Bojana, Stopper, Helga, "DNA damage in circulating leukocytes measured with the comet assay may predict the risk of death" in Scientific Reports, 11, no. 1 (2021), https://doi.org/10.1038/s41598-021-95976-7 . .