Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition
Само за регистроване кориснике
2021
Аутори
Ušjak, LjubošMilutinović, Violeta
Đorđić Crnogorac, Marija J.
Stanojković, Tatjana P.
Niketić, Marjan S.
Kukić-Marković, Jelena
Petrović, Silvana
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these ca...rbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.
Кључне речи:
Three wild Pyrus taxa / bark dry extracts / liquid chromatography / biological activity / molecular dockingИзвор:
Chemistry & Biodiversity, 2021, 18, 10Издавач:
- John Wiley and Sons Inc
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200161 (Универзитет у Београду, Фармацеутски факултет) (RS-200161)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200043 (Институт за онкологију и радиологију Србије, Београд) (RS-200043)
DOI: 10.1002/cbdv.202100446
ISSN: 1612-1872
WoS: 000695725700001
Scopus: 2-s2.0-85114306328
Институција/група
PharmacyTY - JOUR AU - Ušjak, Ljuboš AU - Milutinović, Violeta AU - Đorđić Crnogorac, Marija J. AU - Stanojković, Tatjana P. AU - Niketić, Marjan S. AU - Kukić-Marković, Jelena AU - Petrović, Silvana PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3957 AB - Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity. PB - John Wiley and Sons Inc T2 - Chemistry & Biodiversity T1 - Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition VL - 18 IS - 10 DO - 10.1002/cbdv.202100446 ER -
@article{ author = "Ušjak, Ljuboš and Milutinović, Violeta and Đorđić Crnogorac, Marija J. and Stanojković, Tatjana P. and Niketić, Marjan S. and Kukić-Marković, Jelena and Petrović, Silvana", year = "2021", abstract = "Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity.", publisher = "John Wiley and Sons Inc", journal = "Chemistry & Biodiversity", title = "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition", volume = "18", number = "10", doi = "10.1002/cbdv.202100446" }
Ušjak, L., Milutinović, V., Đorđić Crnogorac, M. J., Stanojković, T. P., Niketić, M. S., Kukić-Marković, J.,& Petrović, S.. (2021). Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity John Wiley and Sons Inc., 18(10). https://doi.org/10.1002/cbdv.202100446
Ušjak L, Milutinović V, Đorđić Crnogorac MJ, Stanojković TP, Niketić MS, Kukić-Marković J, Petrović S. Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition. in Chemistry & Biodiversity. 2021;18(10). doi:10.1002/cbdv.202100446 .
Ušjak, Ljuboš, Milutinović, Violeta, Đorđić Crnogorac, Marija J., Stanojković, Tatjana P., Niketić, Marjan S., Kukić-Marković, Jelena, Petrović, Silvana, "Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition" in Chemistry & Biodiversity, 18, no. 10 (2021), https://doi.org/10.1002/cbdv.202100446 . .