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Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition
dc.creator | Ušjak, Ljuboš | |
dc.creator | Milutinović, Violeta | |
dc.creator | Đorđić Crnogorac, Marija J. | |
dc.creator | Stanojković, Tatjana P. | |
dc.creator | Niketić, Marjan S. | |
dc.creator | Kukić-Marković, Jelena | |
dc.creator | Petrović, Silvana | |
dc.date.accessioned | 2021-09-15T10:51:51Z | |
dc.date.available | 2021-09-15T10:51:51Z | |
dc.date.issued | 2021 | |
dc.identifier.issn | 1612-1872 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/3957 | |
dc.description.abstract | Dry MeOH extracts of the twig barks of Pyrus communis subsp. pyraster, P. spinosa and their hybrid P.×jordanovii nothosubsp. velenovskyi, collected in wild in Serbia, were analyzed. By LC/MS, the contents of arbutin (99.9–131.0 mg/g), chlorogenic acid (2.2–6.3 mg/g), catechin (1.0–5.3 mg/g) and total dimeric and trimeric procyanidins (42.2–61.3 mg/g), including procyanidin B2 (8.9–17.2 mg/g), were determined. Colorimetri- cally, high contents of total phenolics (436.2–533.4 mg GAE/g) and tannins (339.4–425.7 mg GAE/g), as well as strong total antioxidant activities (FRAP values 4.5–5.9 mmol Fe2+ /g), and DPPH (SC50 = 6.6–7.1 μg/ml) and hydroxyl radical (SC50 = 447.1–727.7 μg/ml) scavenging abilities were revealed. In vitro, all extracts exhibited notable inhibition of α-amylase (IC50 = 310.8–617.7 μg/ml) and particularly strong inhibition of α-glucosidase (IC50 = 2.1–3.7 μg/ml). Molecular docking predicted that among identified compounds procyanidin B2 is the best inhibitor of these carbohydrate-digesting enzymes. Obtained results showed that the barks of investigated Pyrus hybrid and its parent taxa have similar composition and bioactivity. | |
dc.publisher | John Wiley and Sons Inc | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS// | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200043/RS// | |
dc.rights | restrictedAccess | |
dc.source | Chemistry & Biodiversity | |
dc.subject | Three wild Pyrus taxa | |
dc.subject | bark dry extracts | |
dc.subject | liquid chromatography | |
dc.subject | biological activity | |
dc.subject | molecular docking | |
dc.title | Barks of Three Wild Pyrus Taxa: Phenolic Constituents, Antioxidant Activity, and in Vitro and in Silico Investigations of α-Amylase and α-Glucosidase Inhibition | |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Ушјак, Љубош; Милутиновић, Виолета; Петровић, Силвана; Ђорђић Црногорац, Марија Ј.; Станојковић, Татјана П.; Никетић, Марјан С.; Кукић‐Марковић, Јелена; | |
dc.citation.volume | 18 | |
dc.citation.issue | 10 | |
dc.citation.rank | M22 | |
dc.identifier.wos | 000695725700001 | |
dc.identifier.doi | 10.1002/cbdv.202100446 | |
dc.identifier.scopus | 2-s2.0-85114306328 | |
dc.type.version | publishedVersion |