Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses
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2021
Authors
Javorac, Dragana
Antonijević, Biljana

Anđelković, Milena

Repić, Aleksandra
Bulat, Petar

Buha-Đorđević, Aleksandra

Baralić, Katarina

Đukić-Ćosić, Danijela

Antonić, Tamara

Bulat, Zorica

Article (Published version)

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Exposure to lead (Pb) is still rising concern worldwide, having in mind that even low-dose exposure can induce various harmful effects. Thus, in-depth knowledge of the targets of Pb toxicity and corresponding mechanisms is essential. In the presented study, the six groups (male Wistar rats, n = 6) received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, each day by oral gavage, while the control group received distilled water only. All animals were sacrificed 24 h after the treatment, and blood was collected for the analysis of hematological, biochemical, oxidative status and essential elements levels. An external and internal dose-response relationship was performed using PROASTweb 70.1 software. The results showed that low doses of Pb affect hematological parameters and lipid profile after 28 days. The possible mechanisms at examined Pb dose levels were a decrease in SOD, O2• and Cu and an increase in Zn levels. The dose-dependent nature of changes in cholesterol, HDL c...holesterol, O2.-, SOD, AOPP in serum and hemoglobin, Fe, Zn, Cu in blood were obtained in this study. The most sensitive parameters that were alerted are Cu blood levels (BMDL5: 1.4 ng/kg b.w./day) and SOD activity (BMDL5: 0.5 μg/kg b.w./day). The presented results provide information that may be useful in further assessing the health risks of low-level Pb exposure.
Keywords:
Dose-response relationship / Essential metals / Hematological effects / Low-level Pb dose / Oxidative statusSource:
Environmental Pollution, 2021, 291Publisher:
- Elsevier Ltd
Funding / projects:
DOI: 10.1016/j.envpol.2021.118103
ISSN: 0269-7491
WoS: 000696647500012
Scopus: 2-s2.0-85114685844
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PharmacyTY - JOUR AU - Javorac, Dragana AU - Antonijević, Biljana AU - Anđelković, Milena AU - Repić, Aleksandra AU - Bulat, Petar AU - Buha-Đorđević, Aleksandra AU - Baralić, Katarina AU - Đukić-Ćosić, Danijela AU - Antonić, Tamara AU - Bulat, Zorica PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3962 AB - Exposure to lead (Pb) is still rising concern worldwide, having in mind that even low-dose exposure can induce various harmful effects. Thus, in-depth knowledge of the targets of Pb toxicity and corresponding mechanisms is essential. In the presented study, the six groups (male Wistar rats, n = 6) received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, each day by oral gavage, while the control group received distilled water only. All animals were sacrificed 24 h after the treatment, and blood was collected for the analysis of hematological, biochemical, oxidative status and essential elements levels. An external and internal dose-response relationship was performed using PROASTweb 70.1 software. The results showed that low doses of Pb affect hematological parameters and lipid profile after 28 days. The possible mechanisms at examined Pb dose levels were a decrease in SOD, O2• and Cu and an increase in Zn levels. The dose-dependent nature of changes in cholesterol, HDL cholesterol, O2.-, SOD, AOPP in serum and hemoglobin, Fe, Zn, Cu in blood were obtained in this study. The most sensitive parameters that were alerted are Cu blood levels (BMDL5: 1.4 ng/kg b.w./day) and SOD activity (BMDL5: 0.5 μg/kg b.w./day). The presented results provide information that may be useful in further assessing the health risks of low-level Pb exposure. PB - Elsevier Ltd T2 - Environmental Pollution T1 - Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses VL - 291 DO - 10.1016/j.envpol.2021.118103 ER -
@article{ author = "Javorac, Dragana and Antonijević, Biljana and Anđelković, Milena and Repić, Aleksandra and Bulat, Petar and Buha-Đorđević, Aleksandra and Baralić, Katarina and Đukić-Ćosić, Danijela and Antonić, Tamara and Bulat, Zorica", year = "2021", abstract = "Exposure to lead (Pb) is still rising concern worldwide, having in mind that even low-dose exposure can induce various harmful effects. Thus, in-depth knowledge of the targets of Pb toxicity and corresponding mechanisms is essential. In the presented study, the six groups (male Wistar rats, n = 6) received 0.1; 0.5; 1; 3; 7; 15 mg Pb/kg body weight/day for 28 days, each day by oral gavage, while the control group received distilled water only. All animals were sacrificed 24 h after the treatment, and blood was collected for the analysis of hematological, biochemical, oxidative status and essential elements levels. An external and internal dose-response relationship was performed using PROASTweb 70.1 software. The results showed that low doses of Pb affect hematological parameters and lipid profile after 28 days. The possible mechanisms at examined Pb dose levels were a decrease in SOD, O2• and Cu and an increase in Zn levels. The dose-dependent nature of changes in cholesterol, HDL cholesterol, O2.-, SOD, AOPP in serum and hemoglobin, Fe, Zn, Cu in blood were obtained in this study. The most sensitive parameters that were alerted are Cu blood levels (BMDL5: 1.4 ng/kg b.w./day) and SOD activity (BMDL5: 0.5 μg/kg b.w./day). The presented results provide information that may be useful in further assessing the health risks of low-level Pb exposure.", publisher = "Elsevier Ltd", journal = "Environmental Pollution", title = "Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses", volume = "291", doi = "10.1016/j.envpol.2021.118103" }
Javorac, D., Antonijević, B., Anđelković, M., Repić, A., Bulat, P., Buha-Đorđević, A., Baralić, K., Đukić-Ćosić, D., Antonić, T.,& Bulat, Z.. (2021). Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses. in Environmental Pollution Elsevier Ltd., 291. https://doi.org/10.1016/j.envpol.2021.118103
Javorac D, Antonijević B, Anđelković M, Repić A, Bulat P, Buha-Đorđević A, Baralić K, Đukić-Ćosić D, Antonić T, Bulat Z. Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses. in Environmental Pollution. 2021;291. doi:10.1016/j.envpol.2021.118103 .
Javorac, Dragana, Antonijević, Biljana, Anđelković, Milena, Repić, Aleksandra, Bulat, Petar, Buha-Đorđević, Aleksandra, Baralić, Katarina, Đukić-Ćosić, Danijela, Antonić, Tamara, Bulat, Zorica, "Oxidative stress, metallomics and blood toxicity after subacute low-level lead exposure in Wistar rats: Benchmark dose analyses" in Environmental Pollution, 291 (2021), https://doi.org/10.1016/j.envpol.2021.118103 . .