Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study
Authors
Kanjevac, TatjanaTaso, Ervin
Stefanović, Vladimir
Petković-Curcin, Aleksandra
Supić, Gordana
Marković, Dejan
Đukić, Mirjana
Đuran, Boris
Vojvodić, Danilo
Sculean, Anton
Rakić, Mia
Article (Published version)
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Dental caries and periodontitis are among the most common health conditions that are currently recognized as growing socio-economic problems relating to their increasing prevalence, negative socio-economic impact, and harmful effects on systemic health. So far, the exact effects of caries and standard restorative materials on periodontal inflammatory and oxidative status are not established. The present study aimed to investigate the effect of caries and its restoration using standard temporary and permanent filling materials on a panel of 16 inflammatory and oxidative markers in gingival crevicular fluid (GCF) of periodontally healthy individuals, 7 (D7) and 30 (D30) days post-restoration, while the intact teeth represented the control. One hundred ninety systemically and periodontally healthy patients with occlusal caries underwent standard cavity preparation and restorations with one of six standard temporary or permanent restorative material according to indication and randomizatio...n scheme. Interleukin (IL)-2, IFN- g, IL-12, IL-17A, IL-13, IL-9, IL-10, IL-6, IL-5, IL-4, IL-22, TNF-a, IL1- b, thiobarbituric acid reactive substances, superoxide dismutase, and reduced form of glutathione were measured in GCF samples by flowcytometry and spectrophotometry in aid of commercial diagnostic assays. Caries affected teeth exhibited significantly increased IL-1 b, IL-17, IL- 22, and TBARS and decreased IL-9 concentrations compared to healthy controls. Treatment generally resulted in an increased antioxidant capacity with exception of zinc-polycarboxylate cement showing distinctive inflammatory pattern. Comparison of inflammatory and oxidative profiles in temporary and permanent restorations showed material-specific patterning which was particularly expressed in temporary materials plausibly related to greater caries extension. Caries affected teeth exhibited a balanced inflammatory pattern in GCF, with a general tendency of homeostatic re-establishment following treatment. Restorative materials did not provide specific pathological effects, although some material groups did exhibit significantly elevated levels of inflammatory and oxidative markers compared to healthy controls, while the material-specific patterning was observed as well.
Keywords:
oxidative stress / inflammation / cytokines / caries / dental restoration / periodontitis / periodontium / restorative materialSource:
Frontiers in Immunology, 2021, 12Publisher:
- Frontiers Media S.A.
Funding / projects:
DOI: 10.3389/fimmu.2021.716359
ISSN: 1664-3224
WoS: 000701289100001
Scopus: 2-s2.0-85115911224
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PharmacyTY - JOUR AU - Kanjevac, Tatjana AU - Taso, Ervin AU - Stefanović, Vladimir AU - Petković-Curcin, Aleksandra AU - Supić, Gordana AU - Marković, Dejan AU - Đukić, Mirjana AU - Đuran, Boris AU - Vojvodić, Danilo AU - Sculean, Anton AU - Rakić, Mia PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3980 AB - Dental caries and periodontitis are among the most common health conditions that are currently recognized as growing socio-economic problems relating to their increasing prevalence, negative socio-economic impact, and harmful effects on systemic health. So far, the exact effects of caries and standard restorative materials on periodontal inflammatory and oxidative status are not established. The present study aimed to investigate the effect of caries and its restoration using standard temporary and permanent filling materials on a panel of 16 inflammatory and oxidative markers in gingival crevicular fluid (GCF) of periodontally healthy individuals, 7 (D7) and 30 (D30) days post-restoration, while the intact teeth represented the control. One hundred ninety systemically and periodontally healthy patients with occlusal caries underwent standard cavity preparation and restorations with one of six standard temporary or permanent restorative material according to indication and randomization scheme. Interleukin (IL)-2, IFN- g, IL-12, IL-17A, IL-13, IL-9, IL-10, IL-6, IL-5, IL-4, IL-22, TNF-a, IL1- b, thiobarbituric acid reactive substances, superoxide dismutase, and reduced form of glutathione were measured in GCF samples by flowcytometry and spectrophotometry in aid of commercial diagnostic assays. Caries affected teeth exhibited significantly increased IL-1 b, IL-17, IL- 22, and TBARS and decreased IL-9 concentrations compared to healthy controls. Treatment generally resulted in an increased antioxidant capacity with exception of zinc-polycarboxylate cement showing distinctive inflammatory pattern. Comparison of inflammatory and oxidative profiles in temporary and permanent restorations showed material-specific patterning which was particularly expressed in temporary materials plausibly related to greater caries extension. Caries affected teeth exhibited a balanced inflammatory pattern in GCF, with a general tendency of homeostatic re-establishment following treatment. Restorative materials did not provide specific pathological effects, although some material groups did exhibit significantly elevated levels of inflammatory and oxidative markers compared to healthy controls, while the material-specific patterning was observed as well. PB - Frontiers Media S.A. T2 - Frontiers in Immunology T1 - Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study VL - 12 DO - 10.3389/fimmu.2021.716359 ER -
@article{ author = "Kanjevac, Tatjana and Taso, Ervin and Stefanović, Vladimir and Petković-Curcin, Aleksandra and Supić, Gordana and Marković, Dejan and Đukić, Mirjana and Đuran, Boris and Vojvodić, Danilo and Sculean, Anton and Rakić, Mia", year = "2021", abstract = "Dental caries and periodontitis are among the most common health conditions that are currently recognized as growing socio-economic problems relating to their increasing prevalence, negative socio-economic impact, and harmful effects on systemic health. So far, the exact effects of caries and standard restorative materials on periodontal inflammatory and oxidative status are not established. The present study aimed to investigate the effect of caries and its restoration using standard temporary and permanent filling materials on a panel of 16 inflammatory and oxidative markers in gingival crevicular fluid (GCF) of periodontally healthy individuals, 7 (D7) and 30 (D30) days post-restoration, while the intact teeth represented the control. One hundred ninety systemically and periodontally healthy patients with occlusal caries underwent standard cavity preparation and restorations with one of six standard temporary or permanent restorative material according to indication and randomization scheme. Interleukin (IL)-2, IFN- g, IL-12, IL-17A, IL-13, IL-9, IL-10, IL-6, IL-5, IL-4, IL-22, TNF-a, IL1- b, thiobarbituric acid reactive substances, superoxide dismutase, and reduced form of glutathione were measured in GCF samples by flowcytometry and spectrophotometry in aid of commercial diagnostic assays. Caries affected teeth exhibited significantly increased IL-1 b, IL-17, IL- 22, and TBARS and decreased IL-9 concentrations compared to healthy controls. Treatment generally resulted in an increased antioxidant capacity with exception of zinc-polycarboxylate cement showing distinctive inflammatory pattern. Comparison of inflammatory and oxidative profiles in temporary and permanent restorations showed material-specific patterning which was particularly expressed in temporary materials plausibly related to greater caries extension. Caries affected teeth exhibited a balanced inflammatory pattern in GCF, with a general tendency of homeostatic re-establishment following treatment. Restorative materials did not provide specific pathological effects, although some material groups did exhibit significantly elevated levels of inflammatory and oxidative markers compared to healthy controls, while the material-specific patterning was observed as well.", publisher = "Frontiers Media S.A.", journal = "Frontiers in Immunology", title = "Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study", volume = "12", doi = "10.3389/fimmu.2021.716359" }
Kanjevac, T., Taso, E., Stefanović, V., Petković-Curcin, A., Supić, G., Marković, D., Đukić, M., Đuran, B., Vojvodić, D., Sculean, A.,& Rakić, M.. (2021). Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study. in Frontiers in Immunology Frontiers Media S.A.., 12. https://doi.org/10.3389/fimmu.2021.716359
Kanjevac T, Taso E, Stefanović V, Petković-Curcin A, Supić G, Marković D, Đukić M, Đuran B, Vojvodić D, Sculean A, Rakić M. Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study. in Frontiers in Immunology. 2021;12. doi:10.3389/fimmu.2021.716359 .
Kanjevac, Tatjana, Taso, Ervin, Stefanović, Vladimir, Petković-Curcin, Aleksandra, Supić, Gordana, Marković, Dejan, Đukić, Mirjana, Đuran, Boris, Vojvodić, Danilo, Sculean, Anton, Rakić, Mia, "Estimating the Effects of Dental Caries and Its Restorative Treatment on Periodontal Inflammatory and Oxidative Status: A Short Controlled Longitudinal Study" in Frontiers in Immunology, 12 (2021), https://doi.org/10.3389/fimmu.2021.716359 . .