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Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention
The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that signifi...cant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue.
TY - JOUR
AU - Baralić, Katarina
AU - Božić, Dragica
AU - Živančević, Katarina
AU - Milenković, Milan
AU - Javorac, Dragana
AU - Marić, Đurđica
AU - Antonijević-Miljaković, Evica
AU - Buha-Đorđević, Aleksandra
AU - Vukomanović, Predrag
AU - Ćurčić, Marijana
AU - Bulat, Zorica
AU - Antonijević, Biljana
AU - Đukić-Ćosić, Danijela
PY - 2021
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3996
AB - The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue.
PB - Elsevier Ltd
T2 - Food and Chemical Toxicology
T1 - Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention
VL - 158
DO - 10.1016/j.fct.2021.112671
ER -
@article{
author = "Baralić, Katarina and Božić, Dragica and Živančević, Katarina and Milenković, Milan and Javorac, Dragana and Marić, Đurđica and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Vukomanović, Predrag and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2021",
abstract = "The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue.",
publisher = "Elsevier Ltd",
journal = "Food and Chemical Toxicology",
title = "Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention",
volume = "158",
doi = "10.1016/j.fct.2021.112671"
}
Baralić, K., Božić, D., Živančević, K., Milenković, M., Javorac, D., Marić, Đ., Antonijević-Miljaković, E., Buha-Đorđević, A., Vukomanović, P., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2021). Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention. in Food and Chemical Toxicology
Elsevier Ltd., 158.
https://doi.org/10.1016/j.fct.2021.112671
Baralić K, Božić D, Živančević K, Milenković M, Javorac D, Marić Đ, Antonijević-Miljaković E, Buha-Đorđević A, Vukomanović P, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention. in Food and Chemical Toxicology. 2021;158.
doi:10.1016/j.fct.2021.112671 .
Baralić, Katarina, Božić, Dragica, Živančević, Katarina, Milenković, Milan, Javorac, Dragana, Marić, Đurđica, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Vukomanović, Predrag, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Integrating in silico with in vivo approach to investigate phthalate and
bisphenol A mixture-linked asthma development: Positive
probiotic intervention" in Food and Chemical Toxicology, 158 (2021),
https://doi.org/10.1016/j.fct.2021.112671 . .
