Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention
Samo za registrovane korisnike
2021
Autori
Baralić, KatarinaBožić, Dragica
Živančević, Katarina
Milenković, Milan
Javorac, Dragana
Marić, Đurđica
Antonijević-Miljaković, Evica
Buha-Đorđević, Aleksandra
Vukomanović, Predrag
Ćurčić, Marijana
Bulat, Zorica
Antonijević, Biljana
Đukić-Ćosić, Danijela
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that signifi...cant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue.
Ključne reči:
Probiotic / Endocrine disruptors / Toxicogenomic data mining / Asthma / MechanismsIzvor:
Food and Chemical Toxicology, 2021, 158Izdavač:
- Elsevier Ltd
Finansiranje / projekti:
- Ministarstvo nauke, tehnološkog razvoja i inovacija Republike Srbije, institucionalno finansiranje - 200161 (Univerzitet u Beogradu, Farmaceutski fakultet) (RS-MESTD-inst-2020-200161)
DOI: 10.1016/j.fct.2021.112671
ISSN: 0278-6915
WoS: 000721173800001
Scopus: 2-s2.0-85119294261
Institucija/grupa
PharmacyTY - JOUR AU - Baralić, Katarina AU - Božić, Dragica AU - Živančević, Katarina AU - Milenković, Milan AU - Javorac, Dragana AU - Marić, Đurđica AU - Antonijević-Miljaković, Evica AU - Buha-Đorđević, Aleksandra AU - Vukomanović, Predrag AU - Ćurčić, Marijana AU - Bulat, Zorica AU - Antonijević, Biljana AU - Đukić-Ćosić, Danijela PY - 2021 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3996 AB - The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue. PB - Elsevier Ltd T2 - Food and Chemical Toxicology T1 - Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention VL - 158 DO - 10.1016/j.fct.2021.112671 ER -
@article{ author = "Baralić, Katarina and Božić, Dragica and Živančević, Katarina and Milenković, Milan and Javorac, Dragana and Marić, Đurđica and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Vukomanović, Predrag and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela", year = "2021", abstract = "The aim of this study was to explore the mechanisms of bis(2- ethylhexyl) phthalate (DEHP), dibutyl phthalate (DBP) and bisphenol A (BPA) mixture-induced asthma development and test probiotic as a potential positive intervention. Comparative Toxicogenomics Database (CTD) and ToppGene Suite were used as the main tools for in silico analysis. In vivo 28-day experiment was conducted on rats - seven groups (n = 6): (1) Control: corn oil, (2) P: probiotic (8.78 * 108 CFU/kg/day); (3) DEHP: 50 mg/kg b.w./day, (4) DBP: 50 mg/kg b.w./day, (5) BPA: 25 mg/kg b.w./day; (6) MIX: DEHP + DBP + BPA; (7) MIX + P. Lungs, thymus and kidneys were extracted and prepared for redox status and essential metals analysis. By conducting additional in vitro experiment, probiotic phthalate and BPA binding ability was explored. There were 24 DEHP, DBP and BPA asthma-related genes, indicating the three most probable mechanisms - apoptosis, inflammation and oxidative stress. In vivo experiment confirmed that significant changes in redox status/essential metal parameters were either prominent, or only present in the MIX group, indicating possible additive effects. In vitro experiment confirmed the ability of the multy-strain probiotic to bind DEHP/DBP/BPA mixture, while probiotic administration ameliorated mixtureinduced changes in rat tissue.", publisher = "Elsevier Ltd", journal = "Food and Chemical Toxicology", title = "Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention", volume = "158", doi = "10.1016/j.fct.2021.112671" }
Baralić, K., Božić, D., Živančević, K., Milenković, M., Javorac, D., Marić, Đ., Antonijević-Miljaković, E., Buha-Đorđević, A., Vukomanović, P., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2021). Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention. in Food and Chemical Toxicology Elsevier Ltd., 158. https://doi.org/10.1016/j.fct.2021.112671
Baralić K, Božić D, Živančević K, Milenković M, Javorac D, Marić Đ, Antonijević-Miljaković E, Buha-Đorđević A, Vukomanović P, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention. in Food and Chemical Toxicology. 2021;158. doi:10.1016/j.fct.2021.112671 .
Baralić, Katarina, Božić, Dragica, Živančević, Katarina, Milenković, Milan, Javorac, Dragana, Marić, Đurđica, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Vukomanović, Predrag, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Integrating in silico with in vivo approach to investigate phthalate and bisphenol A mixture-linked asthma development: Positive probiotic intervention" in Food and Chemical Toxicology, 158 (2021), https://doi.org/10.1016/j.fct.2021.112671 . .