Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods
Authors
Gajić, Ina
Kabić, Jovana

Kekić, Dušan
Jovićević, Miloš
Milenković, Marina

Mitić-Ćulafić, Dragana

Trudić, Anika
Ranin, Lazar
Opavski, Nataša
Article (Published version)
Metadata
Show full item recordAbstract
Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical p...ractice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR.
Keywords:
antimicrobial resistance / antimicrobial susceptibility testing / methodsSource:
Antibiotics, 2022, 11, 4Publisher:
- MDPI
DOI: 10.3390/antibiotics11040427
ISSN: 2079-6382
WoS: 000785481400001
Scopus: 2-s2.0-85127690729
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Institution/Community
PharmacyTY - JOUR AU - Gajić, Ina AU - Kabić, Jovana AU - Kekić, Dušan AU - Jovićević, Miloš AU - Milenković, Marina AU - Mitić-Ćulafić, Dragana AU - Trudić, Anika AU - Ranin, Lazar AU - Opavski, Nataša PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4083 AB - Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical practice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR. PB - MDPI T2 - Antibiotics T1 - Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods VL - 11 IS - 4 DO - 10.3390/antibiotics11040427 ER -
@article{ author = "Gajić, Ina and Kabić, Jovana and Kekić, Dušan and Jovićević, Miloš and Milenković, Marina and Mitić-Ćulafić, Dragana and Trudić, Anika and Ranin, Lazar and Opavski, Nataša", year = "2022", abstract = "Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical practice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR.", publisher = "MDPI", journal = "Antibiotics", title = "Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods", volume = "11", number = "4", doi = "10.3390/antibiotics11040427" }
Gajić, I., Kabić, J., Kekić, D., Jovićević, M., Milenković, M., Mitić-Ćulafić, D., Trudić, A., Ranin, L.,& Opavski, N.. (2022). Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods. in Antibiotics MDPI., 11(4). https://doi.org/10.3390/antibiotics11040427
Gajić I, Kabić J, Kekić D, Jovićević M, Milenković M, Mitić-Ćulafić D, Trudić A, Ranin L, Opavski N. Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods. in Antibiotics. 2022;11(4). doi:10.3390/antibiotics11040427 .
Gajić, Ina, Kabić, Jovana, Kekić, Dušan, Jovićević, Miloš, Milenković, Marina, Mitić-Ćulafić, Dragana, Trudić, Anika, Ranin, Lazar, Opavski, Nataša, "Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods" in Antibiotics, 11, no. 4 (2022), https://doi.org/10.3390/antibiotics11040427 . .