Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts
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2022
Authors
Pavlović, IvanRadenković, Mirjana
Branković, Suzana
Milenković, Marina

Niketić, Marjan

Ušjak, Ljuboš

Petrović, Silvana

Article (Published version)

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Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/k...g p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.
Keywords:
antioxidants / Ferula heuffelii dry MeOH extract / gastroprotective effect / liquid chromatography / spasmolytic activitySource:
Chemistry and Biodiversity, 2022, 19, 5Publisher:
- John Wiley and Sons Inc
Funding / projects:
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200161 (University of Belgrade, Faculty of Pharmacy) (RS-200161)
- Ministry of Education, Science and Technological Development, Republic of Serbia, Grant no. 200113 (Univeristy of Niš, Faculty of Medicine) (RS-200113)
DOI: 10.1002/cbdv.202200047
ISSN: 1612-1872
WoS: 000781617100001
Scopus: 2-s2.0-85127965760
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PharmacyTY - JOUR AU - Pavlović, Ivan AU - Radenković, Mirjana AU - Branković, Suzana AU - Milenković, Marina AU - Niketić, Marjan AU - Ušjak, Ljuboš AU - Petrović, Silvana PY - 2022 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4084 AB - Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints. PB - John Wiley and Sons Inc T2 - Chemistry and Biodiversity T1 - Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts VL - 19 IS - 5 DO - 10.1002/cbdv.202200047 ER -
@article{ author = "Pavlović, Ivan and Radenković, Mirjana and Branković, Suzana and Milenković, Marina and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana", year = "2022", abstract = "Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.", publisher = "John Wiley and Sons Inc", journal = "Chemistry and Biodiversity", title = "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts", volume = "19", number = "5", doi = "10.1002/cbdv.202200047" }
Pavlović, I., Radenković, M., Branković, S., Milenković, M., Niketić, M., Ušjak, L.,& Petrović, S.. (2022). Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity John Wiley and Sons Inc., 19(5). https://doi.org/10.1002/cbdv.202200047
Pavlović I, Radenković M, Branković S, Milenković M, Niketić M, Ušjak L, Petrović S. Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity. 2022;19(5). doi:10.1002/cbdv.202200047 .
Pavlović, Ivan, Radenković, Mirjana, Branković, Suzana, Milenković, Marina, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts" in Chemistry and Biodiversity, 19, no. 5 (2022), https://doi.org/10.1002/cbdv.202200047 . .