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dc.creatorBufan, Biljana
dc.creatorArsenović-Ranin, Nevena
dc.creatorŽivković, Irena
dc.creatorPetrović, Raisa
dc.creatorLeposavić, Gordana
dc.date.accessioned2022-05-24T08:55:34Z
dc.date.available2022-05-24T08:55:34Z
dc.date.issued2022
dc.identifier.issn0024-3205
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/4109
dc.description.abstractAims: Given that deprivation of noradrenaline acting on lymphocytes through β-adrenoceptor influences anti- body response, the effects of propranolol treatment beginning two days before immunization with quadrivalent inactivated influenza vaccine (QIV) on IgG response and underlying cellular molecular mechanism in mice were investigated. Main methods: Twenty-one days post-immunization the total QIV antigen-specific IgG titer and IgG subclass titers in sera were determined using ELISA. Additionally, the total counts of germinal centre (GC) B cells, T follicular helper (Tfh) and T follicular regulatory (Tfr) cells in draining lymph nodes (dLNs) and spleens, in vitro prolif- eration of interacting B cells and Th cells and IL-21 synthesis in Th cells in response to QIV antigens and/or mitogen were attested using flow cytometry analysis. In QIV antigen-stimulated dLN cell and splenocyte cultures were also measured concentrations of INF-γ and IL-4, cytokines upregulating IgG2a and IgG1 synthesis, respectively. Key findings: Propranolol decreased the total QIV antigen-specific IgG titer. This correlated with lower GC B cell count and the shift in Tfr/Tfh cell and Tfr/GC B cell ratio towards Tfr in propranolol-treated mice compared with controls. Consistently, QIV antigen-stimulated proliferation of B cells and Th cells from propranolol-treated mice in vitro was impaired. This correlated with the lower frequency of QIV antigen-specific IL-21-producing cells among Th cells. Additionally, in propranolol-treated mice, in accordance with the changes in INF-γ/IL-4 ratio in dLN cell/splenocyte cultures, serum IgG2a/IgG1 ratio was shifted towards IgG1 reflecting decreased IgG2a response. Significance: The study indicates that chronic propranolol treatment may impair response to QIV.
dc.publisherElsevier Inc.
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200177/RS//
dc.rightsrestrictedAccess
dc.sourceLife Sciences
dc.subjectAntibody response
dc.subjectIgG subclass profile
dc.subjectQIV
dc.subjectTfh cells
dc.subjectTfr cells
dc.subjectTh cell cytokine profile
dc.titleB-cell response to seasonal influenza vaccine in mice is amenable to pharmacological modulation through β-adrenoceptor
dc.typearticle
dc.rights.licenseARR
dc.citation.volume301
dc.citation.rankM21
dc.identifier.wos00082939200000
dc.identifier.doi10.1016/j.lfs.2022.120617
dc.identifier.scopus2-s2.0-85129921034
dc.type.versionpublishedVersion


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