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An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection
dc.creator | Semenova, Irina | |
dc.creator | Bryskina, Diana | |
dc.creator | Cvetanović Kljakić, Aleksandra | |
dc.creator | Ražić, Slavica | |
dc.creator | Ananiev, Vasiliy | |
dc.creator | Rodin, Igor | |
dc.creator | Shpigun, Oleg | |
dc.creator | Stavrianidi, Andrey | |
dc.date.accessioned | 2022-05-26T11:32:32Z | |
dc.date.available | 2022-05-26T11:32:32Z | |
dc.date.issued | 2022 | |
dc.identifier.issn | 0958-0344 | |
dc.identifier.uri | https://onlinelibrary.wiley.com/doi/abs/10.1002/pca.3133 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/4115 | |
dc.description.abstract | Introduction: Limited availability of individual standards is a bottleneck for quality control of functional foods and natural medicines. The use of standard mixtures or secondary standards is a possible alternative in this case. Earlier, an approach known as standardised reference extract (RE) strategy was introduced for HPLC-UV analysis of different plant materials; however, its application in HPLC-MS analysis has not been investigated. Objective: To establish an HPLC-MS-based RE method for determination of ginsenoside content in ginseng infusions using commercially available extract refer- ence material of Panax quinquefolius L. Results: The developed HPLC-MS method was validated as precise (1.1%–9.4% intra-day variation; 1.6%–12.8% inter-day variation) and highly sensitive [limit of detection (LOD): 1–40 ng/mL; limit of quantification (LOQ): 4–120 ng/mL]. The sta- bility of samples was satisfactory (5.7%–16.3%). The RE quantification method was compared with the external standard method, and the obtained difference was not significant, mostly in the range of 5%–10%. Matrix effects for the diluted samples of RE and ginseng infusions, determined via the standard addition method, were in the range of 85%–115% and 80%–126%, respectively, and were also positively corre- lated with the ginsenoside concentration. Eleven batches of ginseng infusions from different manufacturers were analysed using the established method. Conclusion: The method for HPLC-MS-based ginsenoside quantification using RE as a secondary standard was established for the first time. The results of this study dem- onstrate that the application of the standardised RE strategy in HPLC-MS can mini- mise the matrix effect-related error in addition to the cost-effective quality control of herbal products, foods, and traditional medicines. | |
dc.publisher | John Wiley and Sons Ltd | |
dc.relation | info:eu-repo/grantAgreement/MESTD/inst-2020/200161/RS// | |
dc.relation | Serbian Ministry of Education, Science, and Technological Development (contract number: 451-03-820/2019-14). | |
dc.rights | restrictedAccess | |
dc.source | Phytochemical Analysis | |
dc.subject | ginsenosides | |
dc.subject | HPLC-MS | |
dc.subject | method validation | |
dc.subject | quality control | |
dc.subject | reference extract | |
dc.title | An application of the standardised reference extract quantification strategy in the quality control of ginseng infusions by liquid chromatography with mass spectrometric detection | |
dc.type | article | |
dc.rights.license | ARR | |
dc.citation.volume | 33 | |
dc.citation.issue | 6 | |
dc.citation.spage | 838 | |
dc.citation.epage | 850 | |
dc.citation.rank | M21 | |
dc.identifier.wos | 00079354760000 | |
dc.identifier.doi | 10.1002/pca.3133 | |
dc.identifier.scopus | 2-s2.0-85129802205 | |
dc.type.version | publishedVersion |