Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography
Само за регистроване кориснике
2003
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
This paper describes a simple, rapid, and reliable HPTLC procedure for the determination of cyproterone acetate and ethinylestradiol in tablets. Identification and determination were performed on 10 cm x 20 cm, LiChrospher silica gel 60 F-254 HPTLC plates with cyclohexane-ethyl acetate, 6 + 4 (nu/nu), as mobile phase. Calibration plots were established showing the dependence of response (peak area) on amount chromatographed. The ranges validated were 250-4000 ng (r = 0.9923) and 100-500 ng (r = 0.9996) for cyproterone acetate and ethinylestradiol, respectively. The suitability of this HPTLC method for quantitative determination of the compounds was proved by validation of precision and accuracy. The method was used for determination of the compounds in commercial pharmaceutical dosage forms (Androcur 10 mg and Diane 35 tablets). The method is simple, reproducible, and accurate and can be used as a more effective option than other chromatographic techniques in routine quality control.
Извор:
Journal of Planar Chromatography - Modern TLC, 2003, 16, 1, 45-47Издавач:
- Research Inst Medicinal Plants, Budakalasz
DOI: 10.1556/JPC.16.2003.1.9
ISSN: 0933-4173
WoS: 000182564900009
Scopus: 2-s2.0-0037260170
Институција/група
PharmacyTY - JOUR AU - Pavić, K AU - Čudina, Olivera AU - Agbaba, Danica AU - Vladimirov, S PY - 2003 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/416 AB - This paper describes a simple, rapid, and reliable HPTLC procedure for the determination of cyproterone acetate and ethinylestradiol in tablets. Identification and determination were performed on 10 cm x 20 cm, LiChrospher silica gel 60 F-254 HPTLC plates with cyclohexane-ethyl acetate, 6 + 4 (nu/nu), as mobile phase. Calibration plots were established showing the dependence of response (peak area) on amount chromatographed. The ranges validated were 250-4000 ng (r = 0.9923) and 100-500 ng (r = 0.9996) for cyproterone acetate and ethinylestradiol, respectively. The suitability of this HPTLC method for quantitative determination of the compounds was proved by validation of precision and accuracy. The method was used for determination of the compounds in commercial pharmaceutical dosage forms (Androcur 10 mg and Diane 35 tablets). The method is simple, reproducible, and accurate and can be used as a more effective option than other chromatographic techniques in routine quality control. PB - Research Inst Medicinal Plants, Budakalasz T2 - Journal of Planar Chromatography - Modern TLC T1 - Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography VL - 16 IS - 1 SP - 45 EP - 47 DO - 10.1556/JPC.16.2003.1.9 ER -
@article{ author = "Pavić, K and Čudina, Olivera and Agbaba, Danica and Vladimirov, S", year = "2003", abstract = "This paper describes a simple, rapid, and reliable HPTLC procedure for the determination of cyproterone acetate and ethinylestradiol in tablets. Identification and determination were performed on 10 cm x 20 cm, LiChrospher silica gel 60 F-254 HPTLC plates with cyclohexane-ethyl acetate, 6 + 4 (nu/nu), as mobile phase. Calibration plots were established showing the dependence of response (peak area) on amount chromatographed. The ranges validated were 250-4000 ng (r = 0.9923) and 100-500 ng (r = 0.9996) for cyproterone acetate and ethinylestradiol, respectively. The suitability of this HPTLC method for quantitative determination of the compounds was proved by validation of precision and accuracy. The method was used for determination of the compounds in commercial pharmaceutical dosage forms (Androcur 10 mg and Diane 35 tablets). The method is simple, reproducible, and accurate and can be used as a more effective option than other chromatographic techniques in routine quality control.", publisher = "Research Inst Medicinal Plants, Budakalasz", journal = "Journal of Planar Chromatography - Modern TLC", title = "Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography", volume = "16", number = "1", pages = "45-47", doi = "10.1556/JPC.16.2003.1.9" }
Pavić, K., Čudina, O., Agbaba, D.,& Vladimirov, S.. (2003). Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography. in Journal of Planar Chromatography - Modern TLC Research Inst Medicinal Plants, Budakalasz., 16(1), 45-47. https://doi.org/10.1556/JPC.16.2003.1.9
Pavić K, Čudina O, Agbaba D, Vladimirov S. Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography. in Journal of Planar Chromatography - Modern TLC. 2003;16(1):45-47. doi:10.1556/JPC.16.2003.1.9 .
Pavić, K, Čudina, Olivera, Agbaba, Danica, Vladimirov, S, "Quantitative analysis of cyproterone acetate and ethinylestradiol in tablets by use of planar chromatography" in Journal of Planar Chromatography - Modern TLC, 16, no. 1 (2003):45-47, https://doi.org/10.1556/JPC.16.2003.1.9 . .