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dc.creatorUšjak, Dušan
dc.creatorNovović, Katarina
dc.creatorFilipić, Brankica
dc.creatorKojić, Milan
dc.creatorFilipović, Nenad
dc.creatorStevanović, Magdalena
dc.creatorMilenković, Marina
dc.date.accessioned2022-06-21T10:27:27Z
dc.date.available2022-06-21T10:27:27Z
dc.date.issued2022
dc.identifier.issn1364-5072
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/4177
dc.description.abstractAims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
dc.publisherJohn Wiley and Sons Inc
dc.relationinfo:eu-repo/grantAgreement/MESTD/Basic Research (BR or ON)/173019/RS//
dc.relationinfo:eu-repo/grantAgreement/MESTD/inst-2020/200175/RS//
dc.rightsrestrictedAccess
dc.sourceJournal of Applied Microbiology
dc.subjectWGS
dc.subjectAc. baumannii
dc.subjectcolistin
dc.subjectresistance mechanisms
dc.subjectselenium nanoparticles
dc.subjectsynergy
dc.titleIn vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
dc.typearticle
dc.rights.licenseARR
dc.citation.volume133
dc.citation.issue3
dc.citation.spage1197
dc.citation.spage1197
dc.citation.epage1206
dc.citation.rankM22
dc.identifier.wos000806140500001
dc.identifier.doi10.1111/jam.15638
dc.identifier.scopus2-s2.0-85131637011
dc.type.versionpublishedVersion


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