Study on protolytic equilibria of lorazepam and oxazepam by UV and NMR spectroscopy
Abstract
Protolytic equilibria in homogeneous and heterogeneous systems of lorazepam and oxazepam, which are sparingly soluble ampholytes from the class of 1,4-benzodiazepines, were studied at 25 degreesC and ionic strength of 0.1 M. Acidity constants and equilibrium constants in a heterogeneous system were determined. On the basis of the analysis of the corresponding C-13- and H-1-NMR spectra, deprotonation site in the molecules of the investigated compounds was predicted. Finally, the correlation between chemical shifts in the H-1-NMR spectra and the acidity of the amide proton of 1,4-benzodiazepines was established.
Source:
Journal of Pharmaceutical and Biomedical Analysis, 2003, 31, 4, 693-699Publisher:
- Pergamon-Elsevier Science Ltd, Oxford
DOI: 10.1016/S0731-7085(02)00686-6
ISSN: 0731-7085
PubMed: 12644196