Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man
Само за регистроване кориснике
2003
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used t...o determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.
Извор:
Journal of Planar Chromatography - Modern TLC, 2003, 16, 3, 211-213Издавач:
- Research Inst Medicinal Plants, Budakalasz
DOI: 10.1556/JPC.16.2003.3.8
ISSN: 0933-4173
WoS: 000183923100008
Scopus: 2-s2.0-0038489377
Институција/група
PharmacyTY - JOUR AU - Miljković, Branislava AU - Brzaković, B AU - Kovacević, I AU - Agbaba, Danica AU - Pokrajac, Milena PY - 2003 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/419 AB - A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration. PB - Research Inst Medicinal Plants, Budakalasz T2 - Journal of Planar Chromatography - Modern TLC T1 - Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man VL - 16 IS - 3 SP - 211 EP - 213 DO - 10.1556/JPC.16.2003.3.8 ER -
@article{ author = "Miljković, Branislava and Brzaković, B and Kovacević, I and Agbaba, Danica and Pokrajac, Milena", year = "2003", abstract = "A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.", publisher = "Research Inst Medicinal Plants, Budakalasz", journal = "Journal of Planar Chromatography - Modern TLC", title = "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man", volume = "16", number = "3", pages = "211-213", doi = "10.1556/JPC.16.2003.3.8" }
Miljković, B., Brzaković, B., Kovacević, I., Agbaba, D.,& Pokrajac, M.. (2003). Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC Research Inst Medicinal Plants, Budakalasz., 16(3), 211-213. https://doi.org/10.1556/JPC.16.2003.3.8
Miljković B, Brzaković B, Kovacević I, Agbaba D, Pokrajac M. Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC. 2003;16(3):211-213. doi:10.1556/JPC.16.2003.3.8 .
Miljković, Branislava, Brzaković, B, Kovacević, I, Agbaba, Danica, Pokrajac, Milena, "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man" in Journal of Planar Chromatography - Modern TLC, 16, no. 3 (2003):211-213, https://doi.org/10.1556/JPC.16.2003.3.8 . .