A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used t...o determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.
Source:
Journal of Planar Chromatography - Modern TLC, 2003, 16, 3, 211-213
TY - JOUR
AU - Miljković, Branislava
AU - Brzaković, B
AU - Kovacević, I
AU - Agbaba, Danica
AU - Pokrajac, Milena
PY - 2003
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/419
AB - A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.
PB - Research Inst Medicinal Plants, Budakalasz
T2 - Journal of Planar Chromatography - Modern TLC
T1 - Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man
VL - 16
IS - 3
SP - 211
EP - 213
DO - 10.1556/JPC.16.2003.3.8
ER -
@article{
author = "Miljković, Branislava and Brzaković, B and Kovacević, I and Agbaba, Danica and Pokrajac, Milena",
year = "2003",
abstract = "A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.",
publisher = "Research Inst Medicinal Plants, Budakalasz",
journal = "Journal of Planar Chromatography - Modern TLC",
title = "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man",
volume = "16",
number = "3",
pages = "211-213",
doi = "10.1556/JPC.16.2003.3.8"
}
Miljković, B., Brzaković, B., Kovacević, I., Agbaba, D.,& Pokrajac, M.. (2003). Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC
Research Inst Medicinal Plants, Budakalasz., 16(3), 211-213.
https://doi.org/10.1556/JPC.16.2003.3.8
Miljković B, Brzaković B, Kovacević I, Agbaba D, Pokrajac M. Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man. in Journal of Planar Chromatography - Modern TLC. 2003;16(3):211-213.
doi:10.1556/JPC.16.2003.3.8 .
Miljković, Branislava, Brzaković, B, Kovacević, I, Agbaba, Danica, Pokrajac, Milena, "Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man" in Journal of Planar Chromatography - Modern TLC, 16, no. 3 (2003):211-213,
https://doi.org/10.1556/JPC.16.2003.3.8 . .
