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dc.creatorMiljković, Branislava
dc.creatorBrzaković, B
dc.creatorKovacević, I
dc.creatorAgbaba, Danica
dc.creatorPokrajac, Milena
dc.date.accessioned2019-09-02T10:56:37Z
dc.date.available2019-09-02T10:56:37Z
dc.date.issued2003
dc.identifier.issn0933-4173
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/419
dc.description.abstractA thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration.en
dc.publisherResearch Inst Medicinal Plants, Budakalasz
dc.rightsrestrictedAccess
dc.sourceJournal of Planar Chromatography - Modern TLC
dc.titleQuantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in manen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractПокрајац, Милена; Aгбаба, Даница; Брзаковић, Б; Миљковић, Бранислава; Ковацевић, И;
dc.citation.volume16
dc.citation.issue3
dc.citation.spage211
dc.citation.epage213
dc.citation.other16(3): 211-213
dc.citation.rankM23
dc.identifier.wos000183923100008
dc.identifier.doi10.1556/JPC.16.2003.3.8
dc.identifier.scopus2-s2.0-0038489377
dc.identifier.rcubconv_1411
dc.type.versionpublishedVersion


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