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Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man
dc.creator | Miljković, Branislava | |
dc.creator | Brzaković, B | |
dc.creator | Kovacević, I | |
dc.creator | Agbaba, Danica | |
dc.creator | Pokrajac, Milena | |
dc.date.accessioned | 2019-09-02T10:56:37Z | |
dc.date.available | 2019-09-02T10:56:37Z | |
dc.date.issued | 2003 | |
dc.identifier.issn | 0933-4173 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/419 | |
dc.description.abstract | A thin-layer chromatographic (TLC) method for quantitative analysis of nimesulide in human plasma has been developed, validated, and applied to a pharmacokinetic study of healthy volunteers. A previously published method has been modified and simplified (instead of two-step sample preparation a single step extraction procedure was employed) which makes the new method timesaving. Nimesulide plasma concentrations were analyzed after single-step extraction with dichloromethane. The sample preparation procedure described enables efficient extraction - recoveries were 99 +/- 8% (mean +/- SD). Chromatography was performed on silica gel 60 F-254 TLC plates, developed with toluene-acetone, 100 + 10 (nu/nu) and evaluated densitometricatly at lambda = 310 nin in reflectance-absorbance mode. The R-F was 0.5025. The method presented is characterized by high sensitivity (0.1 mg L-1) and within-run and day-to-day coefficients of variation of 2.8-4.5% and 4.8-8.5%, respectively. The method was used to determine the pharmacokinetic profile of nimesulide in healthy volunteers after oral administration. | en |
dc.publisher | Research Inst Medicinal Plants, Budakalasz | |
dc.rights | restrictedAccess | |
dc.source | Journal of Planar Chromatography - Modern TLC | |
dc.title | Quantitative analysis of nimesulide in plasma by thin-layer chromatography: Application to pharmacokinetic studies in man | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Покрајац, Милена; Aгбаба, Даница; Брзаковић, Б; Миљковић, Бранислава; Ковацевић, И; | |
dc.citation.volume | 16 | |
dc.citation.issue | 3 | |
dc.citation.spage | 211 | |
dc.citation.epage | 213 | |
dc.citation.other | 16(3): 211-213 | |
dc.citation.rank | M23 | |
dc.identifier.wos | 000183923100008 | |
dc.identifier.doi | 10.1556/JPC.16.2003.3.8 | |
dc.identifier.scopus | 2-s2.0-0038489377 | |
dc.type.version | publishedVersion |