JavaScript is disabled for your browser. Some features of this site may not work without it.
Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun p...rotection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
TY - JOUR
AU - Martić, Radava
AU - Kotur-Stevuljević, Jelena
AU - Malenović, Anđelija
AU - Ušjak, Ljuboš
AU - Petrović, Silvana
AU - Čalija, Bojan
AU - Milić, Jela
AU - Krajišnik, Danina
PY - 2022
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4200
AB - Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.
PB - SAGE Publications Inc.
T2 - Natural Product Communications
T1 - Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment
VL - 17
IS - 7
DO - 10.1177/1934578X221112811
ER -
@article{
author = "Martić, Radava and Kotur-Stevuljević, Jelena and Malenović, Anđelija and Ušjak, Ljuboš and Petrović, Silvana and Čalija, Bojan and Milić, Jela and Krajišnik, Danina",
year = "2022",
abstract = "Fast inverted, oil-in-water (o/w) emulsions, also known as SWitch-Oil-Phase (SWOP) emulsions, express the performances of both o/w and water-in-oil (w/o) emulsions during application to the skin, favoring their use as cosmetic carriers in sunscreen products. The objective of this study was to investigate the antioxidant potential (by 2 different methods) and the ultraviolet (UV) absorption ability of SWOP emulsion (S) with incorporated plant-based antioxidants dihydroquercetin (DHQ) and β-carotene (βC), using quercetin (Q) in a reference emulsion, in addition to the evaluation of their physicochemical properties and stability. A new biochemical extra- cellular model for in vitro assessment of antioxidative properties for the SWOP emulsions (S, SQ, SDHQ, and SDHQβC) was developed and compared with the results of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The analyses were performed at 20 °C and 37 °C, and oxidative stress parameters were monitored and statistically analyzed. The sun protection factor (SPF) of the samples was determined in vitro. Q and DHQ incorporated into the SWOP emulsion exhibited a strong DPPH radical scavenging ability. Neither incorporated nor pure βC showed DPPH radical scavenging ability at the tested concentrations. Contrary to that, in the bioenvironment conditions, SDHQβC showed minor antioxidative effects increase and also a significant decrease in exogenous pro-oxidative effects, caused by pro-oxidant, when compared to SDHQ. The obtained SPFs of SDHQβC, SDHQ, and SQ were 5.19, 4.65, and 3.35, respectively. The phys- icochemical stability of the emulsions was satisfactory during 1 month storage. The presented results demonstrated that the SWOP emul- sion is a suitable carrier for antioxidants with a photoprotective ability. The novel biochemical approach could be used in addition to DPPH assay with several advantages, relevant for the testing of antioxidant activity of potential active ingredients in cosmetic products.",
publisher = "SAGE Publications Inc.",
journal = "Natural Product Communications",
title = "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment",
volume = "17",
number = "7",
doi = "10.1177/1934578X221112811"
}
Martić, R., Kotur-Stevuljević, J., Malenović, A., Ušjak, L., Petrović, S., Čalija, B., Milić, J.,& Krajišnik, D.. (2022). Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications
SAGE Publications Inc.., 17(7).
https://doi.org/10.1177/1934578X221112811
Martić R, Kotur-Stevuljević J, Malenović A, Ušjak L, Petrović S, Čalija B, Milić J, Krajišnik D. Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment. in Natural Product Communications. 2022;17(7).
doi:10.1177/1934578X221112811 .
Martić, Radava, Kotur-Stevuljević, Jelena, Malenović, Anđelija, Ušjak, Ljuboš, Petrović, Silvana, Čalija, Bojan, Milić, Jela, Krajišnik, Danina, "Fast Inverted Photoprotective o/w Emulsions Loaded With Dihydroquercetin and β-Carotene: An Innovative Approach to In Vitro Assessment of Antioxidant Activity in a Bioenvironment" in Natural Product Communications, 17, no. 7 (2022),
https://doi.org/10.1177/1934578X221112811 . .
