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dc.creatorParojčić, Jelena
dc.creatorKarljiković-Rajić, Katarina
dc.creatorĐurić, Zorica
dc.creatorJovanović, M
dc.creatorIbrić, Svetlana
dc.date.accessioned2019-09-02T10:56:40Z
dc.date.available2019-09-02T10:56:40Z
dc.date.issued2003
dc.identifier.issn0142-2782
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/422
dc.description.abstractParacetamol is a widely used nonsalicylate analgesic and antipyretic drug. The existing methods for the determination of paracetamol in biological fluids are mainly HPLC techniques, although there are some reported methods based on spectrophotometric determinations. However, all these methods involve some extraction or derivatisation procedures. In the present study the UV spectra of investigated samples were recorded over the wavelength range 220-400 nm (lambda step 0.21 nm; scan speed 60 nm/min) and second-order derivative spectra were calculated. Second-order derivative spectra of different blank urine samples displayed the presence of a zero-crossing point at 245-247 nm defined as lambda(zc). The zero-order absorption spectra of paracetamol in water displays maximum absorbance at 243 nm, while in second derivative spectra, a minimum peak at 246 nm was observed. Therefore, the application of zero-crossing technique to the second-derivative UV absorption spectrum should be useful for the determination of paracetamol using 2D(lambdazc). The proposed method enables determination of total paracetamol in urine directly and simply by reading the D-2(lambdazc) of the diluted samples. The obtained results were in good accordance with published data on cumulative urinary excretion after per oral administration of paracetamol obtained applying different spectrophotometric methods of determination. It could be useful for biopharmaceutical characterisation of drug products (monitoring of the levels of paracetamol in urine in bioavailability testing, for the evaluation of in vitro-in vivo correlation and screening of different formulations during drug product development). Copyrighten
dc.publisherJohn Wiley & Sons Ltd, Chichester
dc.rightsopenAccess
dc.sourceBiopharmaceutics & Drug Disposition
dc.subjectparacetamolen
dc.subjectderivative UV spectrophotometryen
dc.subjectdetermination in urineen
dc.titleDevelopment of the second-order derivative UV spectrophotometric method for direct determination of paracetamol in urine intended for biopharmaceutical characterisation of drug productsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИбрић, Светлана; Ђурић, Зорица; Јовановић, М; Карљиковић-Рајић, Катарина; Паројчић, Јелена;
dc.citation.volume24
dc.citation.issue7
dc.citation.spage309
dc.citation.epage314
dc.citation.other24(7): 309-314
dc.citation.rankM22
dc.identifier.wos000185847100003
dc.identifier.doi10.1002/bdd.367
dc.identifier.pmid14520684
dc.identifier.scopus2-s2.0-0141928629
dc.identifier.fulltexthttp://farfar.pharmacy.bg.ac.rs//bitstream/id/1955/420.pdf
dc.identifier.rcubconv_1430
dc.type.versionpublishedVersion


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