Optimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.
Source:
Journal of Liquid Chromatography & Related Technologies, 2003, 26, 20, 3401-3412
TY - JOUR
AU - Medenica, Mirjana
AU - Ivanović, D
AU - Marković, S
AU - Malenović, Anđelija
AU - Misljenović, D
PY - 2003
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/423
AB - Optimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.
PB - Marcel Dekker Inc, New York
T2 - Journal of Liquid Chromatography & Related Technologies
T1 - Optimization of an RP-HPLC method for drug control analysis
VL - 26
IS - 20
SP - 3401
EP - 3412
DO - 10.1081/JLC-120025598
ER -
@article{
author = "Medenica, Mirjana and Ivanović, D and Marković, S and Malenović, Anđelija and Misljenović, D",
year = "2003",
abstract = "Optimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.",
publisher = "Marcel Dekker Inc, New York",
journal = "Journal of Liquid Chromatography & Related Technologies",
title = "Optimization of an RP-HPLC method for drug control analysis",
volume = "26",
number = "20",
pages = "3401-3412",
doi = "10.1081/JLC-120025598"
}
Medenica, M., Ivanović, D., Marković, S., Malenović, A.,& Misljenović, D.. (2003). Optimization of an RP-HPLC method for drug control analysis. in Journal of Liquid Chromatography & Related Technologies
Marcel Dekker Inc, New York., 26(20), 3401-3412.
https://doi.org/10.1081/JLC-120025598
Medenica M, Ivanović D, Marković S, Malenović A, Misljenović D. Optimization of an RP-HPLC method for drug control analysis. in Journal of Liquid Chromatography & Related Technologies. 2003;26(20):3401-3412.
doi:10.1081/JLC-120025598 .
Medenica, Mirjana, Ivanović, D, Marković, S, Malenović, Anđelija, Misljenović, D, "Optimization of an RP-HPLC method for drug control analysis" in Journal of Liquid Chromatography & Related Technologies, 26, no. 20 (2003):3401-3412,
https://doi.org/10.1081/JLC-120025598 . .
