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dc.creatorMedenica, Mirjana
dc.creatorIvanović, D
dc.creatorMarković, S
dc.creatorMalenović, Anđelija
dc.creatorMisljenović, D
dc.date.accessioned2019-09-02T10:56:44Z
dc.date.available2019-09-02T10:56:44Z
dc.date.issued2003
dc.identifier.issn1082-6076
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/423
dc.description.abstractOptimization of important conditions for the reversed-phase high-performance liquid chromatographic method was done for the separation of the active ingredients in Marcaine(R) adrenaline injections (bupivacaine hydrochloride 2.5 mg and adrenaline 5.0 mug). Simultaneous influence of several conditions, such as the mobile phase composition, pH of the mobile phase, and temperature, on important chromatographic criteria for the separation, was investigated. The separation factor values defined the optimal conditions, which were confirmed by analysing the appropriate mathematical models. The 3-D graphs, constructed with sixty-four experimental points, were investigated, and the results showed that the optimal separation was achieved with the mobile phase of methanol-water (65: 35 v/v), by adjusting pH to 3.5 and the temperature range from 20degreesC to 30degreesC. The optimized method was validated and the obtained results were statistically evaluated.en
dc.publisherMarcel Dekker Inc, New York
dc.rightsrestrictedAccess
dc.sourceJournal of Liquid Chromatography & Related Technologies
dc.titleOptimization of an RP-HPLC method for drug control analysisen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractМаленовић, Aнђелија; Марковић, С; Мисљеновић, Д; Меденица, Мирјана; Ивановић, Д;
dc.citation.volume26
dc.citation.issue20
dc.citation.spage3401
dc.citation.epage3412
dc.citation.other26(20): 3401-3412
dc.citation.rankM23
dc.identifier.wos000187602000006
dc.identifier.doi10.1081/JLC-120025598
dc.identifier.scopus2-s2.0-0347511816
dc.identifier.rcubconv_1458
dc.type.versionpublishedVersion


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